Rat Anti-Mouse Kappa-FITC

Référence 1170-02

Conditionnement : 0.5mg

Marque : Southern Biotech

Demander plus d'informations

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

More Information
Clone 187.1
Isotype Rat (Sprague Dawley) IgG1κ
Isotype Control Rat IgG1-FITC (KLH/G1-2-2)
Specificity Mouse Kappa
Immunogen MPC-11 immunoglobulin
Conjugate FITC (Fluorescein)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.2 mL or 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Flow Cytometry – Quality tested 4-15
ELISA – Quality tested 2,3
FLISA – Quality tested
Immunohistochemistry-Frozen Sections – Reported in literature 16
Western Blot-Non-Reducing – Reported in literature 4,17,18
Immunoprecipitation – Reported in literature 19
Purification – Reported in literature 20,21
Costimulation – Reported in literature 22

RRID Number AB_2794664
Gene ID 243469 (Mouse)
Gene ID Symbol Igk (Mouse)
Gene ID Aliases kappa
UniProt ID P01837 (Mouse
UniProt Name IGKC_MOUSE (Mouse)
Documentation
Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view

  1. 1. Yelton DE, Desaymard C, Scharff MD. Use of monoclonal anti-mouse immunoglobulin to detect mouse antibodies. Hybridoma. 1981;1:5-11. (Immunogen)
  2. 2. Förster M, Raposo B, Ekman D, Klaczkowska D, Popovic M, Nandakumar KS, et al. Genetic control of antibody production during collagen-induced arthritis development in heterogeneous stock mice. Arthritis Rheum. 2012;64:3594-603. (ELISA)
  3. 3. Raposo B, Dobritzsch D, Ge C, Ekman D, Xu B, Lindh I, et al. Epitope-specific antibody response is controlled by immunoglobulin VH polymorphisms. J Exp Med. 2014;211:405-11. (ELISA)
  4. 4. Nakamura T, Kubagawa H, Cooper MD. Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci USA. 1992;89:8522-6. (FC, WB)
  5. 5. Bertocci B, De Smet A, Berek C, Weill J, Reynaud C. Immunoglobulin κ light chain gene rearrangement is impaired in mice deficient for DNA polymerase mu. Immunity. 2003;19:203-11. (FC)
  6. 6. Heltemes-Harris L, Liu X, Manser T. Progressive surface B cell antigen receptor down-regulation accompanies efficient development of antinuclear antigen B cells to mature, follicular phenotype. J Immunol. 2004;172:823-33. (FC)
  7. 7. Wang Y, Carter RH. CD19 regulates B cell maturation, proliferation, and positive selection in the FDC zone of murine splenic germinal centers. Immunity. 2005;22:749-61. (FC)
  8. 8. Vettermann C, Lutz J, Selg M, Bösl M, Jäck H. Genomic suppression of murine B29/Ig-β promoter-driven transgenes. Eur J Immunol. 2006;36:3324-33. (FC)
  9. 9. Lindsley RC, Thomas M, Srivastava B, Allman D. Generation of peripheral B cells occurs via two spatially and temporally distinct pathways. Blood. 2007;109:2521-8. (FC)
  10. 10. Bai L, Chen Y, He Y, Dai X, Lin X, Wen R, et al. Phospholipase Cγ2 contributes to light-chain gene activation and receptor editing. Mol Cell Biol. 2007;27:5957-67. (FC)
  11. 11. Liu X, Shen S, Manser T. Influence of B cell antigen receptor expression level on pathways of B cell tolerance induction. J Immunol. 2009;182:398-407. (FC)
  12. 12. Gross AJ, Lyandres JR, Panigrahi AK, Prak ET, DeFranco AL. Developmental acquisition of the Lyn-CD22-SHP-1 inhibitory pathway promotes B cell tolerance. J Immunol. 2009;182:5382-92. (FC)
  13. 13. Stumhofer JS, Tait ED, Quinn WJ 3rd, Hosken N, Spudy B, Goenka R, et al. A role for IL-27p28 as an antagonist of gp130-mediated signaling. Nat Immunol. 2010;11:1119-26. (FC)
  14. 14. Corrah TW, Goonetilleke N, Kopycinski J, Deeks SG, Cohen MS, Borrow P, et al. Reappraisal of the relationship between the HIV-1-protective single-nucleotide polymorphism 35 kilobases upstream of the HLA-C gene and surface HLA-C expression. J Virol. 2011;85:3367-74. (FC)
  15. 15. Knoll M, Yanagisawa Y, Simmons S, Engels N, Wienands J, Melchers F, et al. The non-Ig parts of the VpreB and λ5 proteins of the surrogate light chain play opposite roles in the surface representation of the precursor B cell receptor. J Immunol. 2012;188:6010-7. (FC)
  16. 16. Nandakumar KS, Collin M, Olsén A, Nimmerjahn F, Blom AM, Ravetch JV, et al. Endoglycosidase treatment abrogates IgG arthritogenicity: importance of IgG glycosylation in arthritis. Eur J Immunol. 2007;37:2973-82. (IHC-FS)
  17. 17. Naumann I, Kappler R, von Schweinitz D, Debatin K, Fulda S. Bortezomib primes neuroblastoma cells for TRAIL-induced apoptosis by linking the death receptor to the mitochondrial pathway. Clin Cancer Res. 2011;17:3204-18. (WB-NR)
  18. 18. Unterkircher T, Cristofanon S, Vellanki SH, Nonnenmacher L, Karpel-Massler G, Wirtz CR, et al. Bortezomib primes glioblastoma, including glioblastoma stem cells, for TRAIL by increasing tBid stability and mitochondrial apoptosis. Clin Cancer Res. 2011;17:4019-30. (WB-NR)
  19. 19. Campbell KS, Cambier JC. B lymphocyte antigen receptors (mIg) are non-covalently associated with a disulfide linked, inducibly phosphorylated glycoprotein complex. EMBO J. 1990;9:441-8. (IP)
  20. 20. Vainio O, Dunon D, Aïssi F, Dangy J, McNagny KM, Imhof BA. HEMCAM, an adhesion molecule expressed by c-kit+ hemopoietic progenitors. J Cell Biol. 1996;135:1655-68. (Purification)
  21. 21. Tunheim G, Schjetne KW, Fredriksen AB, Sandlie I, Bogen B. Human CD14 is an efficient target for recombinant immunoglobulin vaccine constructs that deliver T cell epitopes. J Leukoc Biol. 2005;77:303-10. (Purification)
  22. 22. Kisielow M, Kisielow J, Capoferri-Sollami G, Karjalainen K. Expression of lymphocyte activation gene 3 (LAG-3) on B cells is induced by T cells. Eur J Immunol. 2005;35:2081-8. (Costim)

Vous serez peut-être également intéressé par les produits suivants :



Référence
Description
Cond.
Prix HT
1175-09
 0.1mg