E-OSEL-RB0003-96T | Rabbit Pg(Progesterone) ELISA Kit Biovalley

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	0.05 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reacitivity	Rabbit
Specificity	This kit recognizes Rabbit Pg in samples.No significant cross-reactivity or interference between Rabbit Pg and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rabbit Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Rabbit Pg are added to the micro ELISA plate wells. Rabbit Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Rabbit Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Progesterone is a hormone secreted by the ovaries, whose main functions include maintaining pregnancy, regulating the menstrual cycle, promoting breast development, and reducing the excitability of the pregnant uterus. When it works together with estrogen, it can promote breast development and prepare for lactation. In addition, progesterone is also used to treat diseases such as threatened miscarriage and habitual miscarriage, premenstrual tension syndrome, anovulatory dysfunctional uterine bleeding, and anovulatory amenorrhea.

Research Area

Signal Transduction

Rabbit Pg(Progesterone) ELISA Kit

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