E-AB-40257-120 | Lamin B1 Polyclonal Antibody Biovalley

Verified Samples	Verified Samples in WB: HepG2, Rat heart, Mouse kidney, Rat brain, Mouse lung, Mouse spleen Verified Samples in IHC: Human breast cancer, Mouse liver, Human uterus, Human liver Verified Samples in IF: HepG2
Dilution	WB 1:250-1:500, IHC 1:200-1:600
Isotype	IgG
Host	Rabbit
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF
Clonality	Polyclonal
Immunogen	Recombinant Rat Lamin-B1 protein expressed by E.coli
Abbre	Lamin B1
Synonyms	ADLD, LMN, LMN2, LMNB, LMNB1, Lamin-B1, Lmnb1, MGC111419, OTTHUMP00000159218, lamin B1
Swissprot	P70615
Calculated MW	67 kDa
Observed MW	72 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Nucleus inner membrane.
Concentration	0.3 mg/mL
Buffer	PBS with 0.02% sodium azide,1% protective protein and 50% glycerol,pH7.4
Purification Method	Antigen Affinity Purification
Research Areas	Cancer, Cell Biology, Signal Transduction, Tags and Cell Markers
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background	The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD).

Cat.No.	Product Name	Sizes
E-AB-1003	Goat Anti-Rabbit IgG(H+L)(peroxidase/HRP conjugated)	500μL , 120μL , 60μL
E-AB-1010	Goat Anti-Rabbit IgG(H+L)(Cyanine3 conjugated)	500μL , 120μL , 60μL
E-AB-1014	Goat Anti-Rabbit IgG(H+L)(FITC conjugated)	500μL , 120μL , 60μL
E-AB-1043	Streptavidin(peroxidase/HRP conjugated)	500μL , 120μL , 60μL
E-AB-1060	Goat Anti-Rabbit IgG(H+L)(Elab Fluor^® 594 conjugated)	500μL , 120μL , 60μL
E-AB-1075	Goat Anti-Rabbit IgG(H+L)(Elab Fluor^® 647 conjugated)	500μL , 120μL , 60μL
E-AB-1099	Elab Fluor^® 405-Goat Anti-Rabbit IgG(H+L)	500μL , 120μL , 1mL
E-AB-1194	HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed	500μL , 120μL , 1mL
E-IR-R217	2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution)	50mL , 18mL , 6mL , 3mL
E-IR-R220	Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0)	10mL , 3mL
E-IR-R221	Super PlusTM Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0)	10mL , 3mL
E-IR-R304A	Western Blot Detection Kit	50Assays
E-IR-R304B	High Accuracy and Absorbability Western Blot Detection Kit	50Assays