Lactate Dehydrogenase (LDH) Assay Kit is an assay kit for the detection and quantification of Lactate Dehydrogenase (LDH) activity in cell lysates using a microplate reader (Cytotoxicity method). Lactate dehydrogenase catalyzes the reaction of lactic acid with NAD+ to produce pyruvic acid and NADH. NADH, under the action of PMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, LDH activity can be quantified by measure the OD value at 450 nm.
Research Articles on Lactate Dehydrogenase (LDH)
Target | Lactate Dehydrogenase (LDH) |
Storage | Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual. |
Validity | The validity for this kit is 12 months. |
Detection Method | Colorimetric |
Sample Type | Cell lysates. |
Availability | Shipped within 5-10 working days. |
Note | This product is for research use only. |