Human IgA Mouse Monoclonal Antibody [Clone ID: NI 69 (A89-034) and NI 184 (A89-035)]

Specifications

Product Data
Clone Name	NI 69 (A89-034) and NI 184 (A89-035)
Applications	ELISA, IF, IP, WB
Recommended Dilution	To identify the presence of IgA in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Immunofluorescence staining of cytoplasmic IgA, and Immunoblotting using a peroxidase labelled monoclonal antibody against TRITC. General Recommended Dilutions: Histochemical Use: 1/20-1/100. Western blot: from 1/200 upwards.
Reactivities	Human
Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Highly purified monoclonal IgA1 isolated from pooled human serum
Specificity	The reactivity of the antiserum is restricted to determinants on the CH2 domain of IgA. It reacts with both subclasses of IgA as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). The antiserum does not react with any other component of the human Ig system or any other human plasma protein as tested. This antiserum has not been tested for cross-reactivity with other species.
Formulation	PBS, pH 7.2 without preservatives or foreign proteins Label: TRITC State: Lyophilized purified Ig fraction Label: Tetramethylrhodamine Isothiocyanate Absorption emission: 554 nm / 573 nm Molar radio: Fluorochrome/IgG ~1.4
Reconstitution Method	Restore by adding 0.5 ml sterile distilled water
Concentration	0.4 mg/ml
Conjugation	TRITC
Storage	Store lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. Avoid Repeated thawing and freezing.