Human Cortisol ELISA Kit

Référence E-OSEL-H0006-96T

Conditionnement : 96T

Marque : Elabscience

Demander plus d'informations

Contactez votre distributeur local :


Téléphone : +1 850 650 7790

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity 2.87 ng/mL
Detection Range 6.25-400 ng/mL
Sample Volume 50 μL
Total Assay Time 1 h 30 min
Reacitivity Human
Specificity This kit recognizes Human Cortisol in samples.No significant cross-reactivity or interference between Human Cortisol and analogues was observed
Recovery 80%-120%
Sample Type serum, plasma, saliva, urine
Detection Method Colorimetric method, ELISA, Competitive
Assay Type Competitive-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 6 months
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Cortisol are added to the micro ELISA plate wells. Human Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF).
Research Area Cell Biology , Metabolism
Other Clones

{{antibodyDetailsPage.numTotal}} Results

Other Formats

{{formatDetailsPage.numTotal}} Results

  • IF:{{item.impact}}

    Journal:{{item.journal}} ({{item.year}})

    DOI:{{item.doi}}

    Reactivity:{{item.species}}

    Sample Type:{{item.organization}}

Species

{{relatedElisaPage.numTotal}} Results

  • Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}

Vous serez peut-être également intéressé par les produits suivants :



Référence
Description
Cond.
Prix HT
E-EL-0029-96T
 96T 
abx051865-96tests
 96tests