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> CF®750 Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL

CF®750 Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, single label for STORM, 1 mg/mL

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Référence 20827-500uL

Conditionnement : 500uL

Marque : Biotium

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Téléphone : +1 850 650 7790

Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, CF® Dye Conjugates, Single Label for STORM

Highly cross-adsorbed donkey anti-mouse IgG (H+L) secondary antibody with single CF® Dye label for STORM super-resolution microscopy.

Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, CF® Dye Conjugates, Single Label for STORM
Antibody Finder

Product Attributes

Antibody reactivity (target)

Mouse IgG
Clonality

Polyclonal
Host species

Donkey
Cross adsorption

Bovine, Chicken, Goat, Guinea pig, Horse, Human, Rabbit, Rat, Sheep, Syrian hamster
Antibody type

Secondary
Concentration

1 mg/mL
Antibody/conjugate formulation

Liquid: PBS/50% glycerol/2 mg/mL BSA/0.05% azide
Species reactivity

Mouse
Secondary/tag antibody applications

IF (cells or tissue sections), STORM
Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Technology

CF® Dyes
Secondary Antibody Product Listings

Tech Tips

Protocol: Fluorescent Western Blotting
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host
Protocols
SDS
Supporting Documents
Download Datasheet

Product Description

This is a highly cross-adsorbed donkey anti-mouse IgG (H L) secondary antibody that has a low degree of labeling (DOL) with one of our CF® Dyes validated for STORM.

  • CF® Dye single label secondary antibody ideal for STORM imaging
  • Highly cross-adsorbed for specific staining with minimal background
  • Available in 9 CF® Dyes validated for STORM

Secondary antibodies with a low DOL, or number of dye molecules per antibody molecule, have been reported to be optimal for STORM (Bittel et al. (2015) Proc. SPIE 9331). This product is prepared by single labeling (DOL=1) of highly cross-adsorbed goat anti-mouse IgG (H L) with a selection of compatible CF® Dyes for (d)-STORM super-resolution microscopy. To minimize cross-reactivity, the antibody has been adsorbed against bovine, chicken, goat, guinea pig, horse, human, rabbit, rat, sheep, and Syrian hamster.

Learn more about CF® Dyes for super-resolution microscopy.

Donkey Anti-Mouse IgG (H+L), Highly Cross-Adsorbed, CF® Dye Conjugates, Single Label for STORM

Conjugation Ex/Em Size Catalog No. Dye Features
CF®505 505/519 nm 50 uL (50 ug) 20878-50uL
0.5 mL (500 ug) 20878-500uL
CF®535ST 535/568 nm 50 uL (50 ug) 20823-50uL CF®535ST Features
0.5 mL (500 ug) 20823-500uL
CF®568 562/583 nm 50 uL (50 ug) 20802-50uL CF®568 Features
0.5 mL (500 ug) 20802-500uL
CF®583R 586/609 nm 50 uL (50 ug) 20794-50uL CF®583R Features
0.5 mL (500 ug) 20794-500uL
CF®597R 597/619 nm 50 uL (50 ug) 20798-50uL CF®597R Features
0.5 mL (500 ug) 20798-500uL
CF®647 650/665 nm 50 uL (50 ug) 20810-50uL CF®647 Features
0.5 mL (500 ug) 20810-500uL
CF®660C 667/685 nm 50 uL (50 ug) 20815-50uL CF®660C Features
0.5 mL (500 ug) 20815-500uL
CF®680 681/698 nm 50 uL (50 ug) 20819-50uL CF®680 Features
0.5 mL (500 ug) 20819-500uL
CF®750 755/777 nm 50 uL (50 ug) 20827-50uL CF®750 Features
0.5 mL (500 ug) 20827-500uL

CF® Dye Secondary Antibodies, Single Label for STORM

Dye Donkey Anti-Goat Donkey Anti-Guinea Pig Donkey Anti-Mouse Donkey Anti-Rabbit Goat Anti-Mouse Goat Anti-Rabbit
Cross-adsorption Ck, GP, Hs, Hu, Ms, Rb, Rt, SHm Bv, Ck, Gt, Hs, Hu, Ms, Rb, Rt, Shp, SHm Bv, Ck, Gt, GP, Hs, Hu, Rb, Rt, Shp, SHm Bv, Ck, Gt, GP, Hs, Hu, Ms, Rt, Shp, SHm Bv, Hs, Hu, Rb, Sw Hu, Ms, Rt
CF®505 20880 20881 20878 20879 20876 20877
CF®535ST 20823 20824 20821 20822
CF®568 20836 20838 20802 20803 20800 20801
CF®583R 20794 20795 20792 20793
CF®597R 20798 20799 20796 20797
CF®647 20829 20837 20810 20811 20808 20809
CF®660C 20815 20816 20812 20813
CF®680 20819 20820 20817 20818
CF®750 20827 20828 20825 20826
Bv: bovine; Ch: chicken; Gt: goat; GP: guinea pig; Hs: horse; Hu: human; Ms: mouse; Rb: rabbit; Sh: sheep; SHm: Syrian hamster; Sw: swine; Rt: rat

View our full selection of bright and specific Secondary Antibodies, or search our catalog using our Antibody Finder. Alternatively, you can view our secondary antibody product listings with catalog numbers.

CF® Dyes offer exceptional brightness and photostability. For more information see our CF® Dye technology page.

Storage and Handling

Liquid format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Liquid format antibodies contain 50% glycerol and will not freeze at -20°C.

Lyophilized format: Store at -20°C, protected from light. Product is stable for at least 6 months from date of receipt when stored as recommended. Reconstitute antibodies in water using the indicated volumes below:

CF® Dye and biotin conjugates: add 0.5 mL dH2O

HRP or DNP conjugates: add 1 mL dH2O

Add the indicated volume of water directly to the vial containing the lyophilized antibody and mix gently to dissolve. Store reconstituted antibody at -20°C and protect from light. Aliquot to avoid repeated freeze/thaw cycles. Alternatively, an equal volume of glycerol can be mixed with the reconstituted antibody so that it will remain liquid at -20°C.

Optional: A preservative such as 0.05% sodium azide (final concentration) can be added to CF® Dye and biotin conjugates. Do not add sodium azide to HRP conjugates.

Note: Storage of the antibody for more than a day at final working dilution is not recommended.

CF is a registered trademark of Biotium, Inc.

References

Download a list of CF® Dye references.

  1. Proc. SPIE 9331, (2015), DOI: 10.1117/12.2083209