Ascorbate Peroxidase (APX) Activity Assay Kit

Référence E-BC-K353-S-100

Conditionnement : 100Assays

Marque : Elabscience

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Detection Principle Ascorbate Peroxidase (APX) can catalyze the reaction between ascorbic acid (ASA) and hydrogen peroxide (H2O2), and ASA can be oxidized to monodehydroascorbic acid (MDASA). The absorbance of solution at 290 nm will decline as the oxidation of ASA. The APX activity can be calculated by detecting the decrease of A290.
Synonyms APX
Sample Type Plant tissue
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (290 nm)
Research Area Plant Physiology Series
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.071 U/g tissue
Detection Range 0.071-47 U/g tissue
Precision inter-assay CV: 6.4% | intra-assay CV: 4.8%
Assay Time 1 h

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
10% Epipremnum aureum tissue homogenization 1
10% Carrot tissue homogenization 1
10% Green pepper tissue homogenization 1
10% Mushrooms tissue homogenization 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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