2×TransTaq®-T PCR SuperMix (-dye)
Référence AS122-01
Conditionnement : 1ml
Marque : TransGen Biotech
TransTaq®-T PCR SuperMix is a ready-to-use mixture of TransTaq®-T DNA Polymerase, dNTPs and optimized buffer. The SuperMix is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O. Efficiency of PCR products with “A” is equal to EasyTaq® DNA polymerase. It is more suitable for high fidelity TA cloning.
• TransTaq®-T PCR SuperMix offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be cloned into pEASY®-T vectors.
• Amplification of genomic DNA fragment up to 8 kb.
Applications
• Complex templates
• TA cloning
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Component | AS122-01/21 | AS122-02/22 |
2×TransTaq®-T PCR SuperMix (-dye)/(+dye) | 1 ml | 5×1 ml |
Nuclease-free Water | 1 ml | 5 ml |
Li D M , Hou Y , Song X P , et al. High Prevalence and Genetic Heterogeneity of Rodent-Borne Bartonella Species on Heixiazi Island, China[J]. Applied and Environmental Microbiology, 2015, 81(23):7981-7992.
Wang J , Lin P , Zhao H , et al. Continuing evolution of canine parvovirus in China: Isolation of novel variants with an Ala5Gly mutation in the VP2 protein.[J]. Infection Genetics & Evolution, 2016, 38:73-78.
Xiang R H , Juan Y , Peng G , et al. Bartonella Species Detected in the Plateau Pikas (Ochotona curzoiae) from Qinghai Plateau in China[J]. Biomedical and Environmental Sciences, 2015, 28(9):674-678.