α-Amanitin [23109-05-9]
Référence A4548-1mg
Conditionnement : 1mg
Marque : APExBIO Technology
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α-Amanitin
α-Amanitin, the most deleterious toxin of A. phalloides to humans, inhibits RNA polymerase II (RNAPII), causing hepatic and renal failure.
- 1. Jing WangWang LiJing Guo, et al. "Reconstitution of chromatin reorganization during mammalian oocyte development." Jul 27, 2024
- 2. Bei Wang, Yu Xu, et al. "Integrating genome-wide CRISPR screens and in silico drug profiling for targeted antidote development." Nat Protoc. 2024 May 30. PMID: 38816517
- 3. Duo Wu, Huang Huang, et al. "The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination." Cell Discov. 2023 Oct 3;9(1):99. PMID: 37789001
- 4. Bei Wang, Arabella H Wan, et al. "Identification of indocyanine green as a STT3B inhibitor against mushroom α-amanitin cytotoxicity." Nat Commun. 2023 May 16;14(1):2241. PMID: 37193694
- 5. Jiangli Lang, Chen Yang, et al. "High Glucose Activates ERK1/2 to Stabilize AP1 and Increase MMP9 Expression in Diabetic Foot Ulcers." Exp Cell Res. 2021 Mar 3;112550. PMID:33675806
- 6. Huo X, Ji L, et al. "The Nuclear Matrix Protein SAFB Cooperates with Major Satellite RNAs to Stabilize Heterochromatin Architecture Partially through Phase Separation." Mol Cell. 2019 Oct 28. pii: S1097-2765(19)30761-0. PMID:31677973
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 918.97 |
| Cas No. | 23109-05-9 |
| Formula | C39H54N10O14S |
| Solubility | ≥1 mg/mL in H2O; Soluble in Ethanol |
| SDF | Download SDF |
| Canonical SMILES | CCC(C)C1C(=O)NCC(=O)NC2CS(=O)C3=C(CC(C(=O)NCC(=O)N1)NC(=O)C(NC(=O)C4CC(CN4C(=O)C(NC2=O)CC(=O)N)O)C(C)C(CO)O)C5=C(N3)C=C(C=C5)O |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment:[1] | |
| Cell lines | Mouse blastocysts |
| Reaction Conditions | 1.1 μg/ml α-amanitin |
| Applications | α-Amanitin inhibited the incorporation of 3H-UMP into embryos. α-Amanitin at the concentration of 1.1 μg/ml was found to inhibit RNA polymerase activity by 32%. |
| Animal experiment:[1] | |
| Animal models | Preimplantation mouse embryos at the two-cell stage of development collected from CF1 female mice |
| Dosage form | 0 ~ 1 μg/ml α-amanitin 48 h |
| Applications | Less than 1% of the embryos which had been treated with 1 μg/ml of α-amanitin developed to the morula or blastocyst stages. α-Amanitin can be used to determine which forms of RNA polymerase are active and the relative amounts of each form which are present in preimplantation mouse embryos. |
| Note | The technical data provided above is for reference only. |
| References: 1. Warner CM, Versteegh LR. In vivo and in vitro effect of alpha-amanitin on preimplantation mouse embryo RNA polymerase. Nature, 1974, 248(5450): 678-680. | |
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