Increase qPCR Sensitivity and Efficiency with PerfectStart® Solutions
Enhance your qPCR results with PerfectStart® Universal Green qPCR SuperMix: a ready-to-use solution delivering high specificity, sensitivity, and efficiency for advanced PCR applications.
TransGen Biotech's PerfectStart® Universal Green qPCR SuperMix is a ready-to-use quantitative PCR (qPCR) cocktail designed for high specificity, sensitivity, and amplification efficiency. The kit contains a PerfectStart® Taq hot-start polymerase (three monoclonal antibodies efficiently bind to the Taq DNA polymerase, effectively blocking DNA polymerase activity and inhibiting non-specific amplification at low temperature), optimized dual-cation buffer, SYBR Green I, dNTPs, PCR enhancer, PCR stabilizer, and universal passive reference dye. qPCR SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers, and nuclease-free water.
Features
Blocking by 3 antibodies, enabling high specificity, high sensitivity, high amplification efficiency, and a wide range of species.
Dual-cation buffer enhances specificity and reduces primer-dimer formation.
This product is premixed with Universal Passive Reference Dye to correct differences in fluorescence detection between wells, without the addition of passive reference dye.
High fluorescence intensity and graceful amplification curve.
The PerfectStart® Universal Green qPCR SuperMix utilizes a universal hot start technique, which enhances the specificity and sensitivity of the PCR reaction. This technique involves adding an inhibitor to the reaction mix, which prevents the polymerase enzyme from being active until the reaction mix is heated to a high temperature. Universal hot start is a common practice in PCR-based applications such as genotyping, gene expression analysis, and DNA sequencing.
