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> XRCC4 Monoclonal Antibody

XRCC4 Monoclonal Antibody

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Cat# E-AB-22044-20

Size : 20uL

Brand : Elabscience

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Phone : +1 850 650 7790

Verified Samples Verified Samples in WB: Hela, 293T
Verified Samples in IHC: Human breast cancer
Verified Samples in IF: Human liver cancer
Dilution WB 1:500-1:2000,  IHC 1:50-300,  IF 1:50-1:200,  IP 1:100-1:300
Isotype IgG
Host Mouse
Reactivity Human
Applications WB,  IHC-p,  IF,  IP
Clonality Monoclonal
Immunogen Synthetic Peptide
Abbre XRCC4
Synonyms DNA double strand break repair and V(D)J recombination protein XRCC4,  DNA repair protein XRCC4,  SSMED,  X ray repair complementing defective repair in Chinese hamster cells 4,  X ray repair cross complementing 4,  X ray repair cross complementing protein 4,  X-ray
Swissprot
Calculated MW 38 kDa
Observed MW 38 kDa

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization Nucleus.
Tissue Specificity Widely expressed
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 0.5% protein protectant and 50% glycerol.
Purification Method Protein A purification
Research Areas Cancer,  Epigenetics and Nuclear Signaling
Clone No. 8K2
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.
Cat.No. Product Name Sizes
E-AB-1001 Goat Anti-Mouse IgG(H+L)(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1011 Goat Anti-Mouse IgG(H+L)(Cyanine3 conjugated) 500μL , 120μL , 60μL
E-AB-1015 Goat Anti-Mouse IgG(H+L)(FITC conjugated) 500μL , 120μL , 60μL
E-AB-1024 Goat Anti-Mouse IgG(H+L)(Biotin conjugated) 500μL , 120μL , 60μL
E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1059 Goat Anti-Mouse IgG(H+L)(Elab Fluor® 594 conjugated) 500μL , 120μL , 60μL
E-AB-1076 Goat Anti-Mouse IgG(H+L)(Elab Fluor® 647 conjugated) 500μL , 120μL , 60μL
E-AB-1196 HRP-Goat Anti-Mouse IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R217 2-step plus Poly-HRP Anti Mouse/Rabbit IgG Detection System (with DAB solution) 50mL , 18mL , 6mL , 3mL
E-IR-R220 Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH9.0) 10mL , 3mL
E-IR-R221 Super Plus™ Highly Sensitive and Rapid Immunohistochemical Kit (pH6.0) 10mL , 3mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Unconjugated

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