Total Amino Acids (T-AA) Colorimetric Assay Kit
Cat# E-BC-K055-M-48T
Size : 48T
Brand : Elabscience
Contact local distributor :
Phone : +1 850 650 7790
| Detection Principle | Copper ions can complex with various amino acids to produce blue-green complex compound, and the depth of color is proportional to the content of total amino acids at a specific wavelength. T-AA content can be calculated with the absorbance at 650 nm. |
| Synonyms | T-AA |
| Sample Type | Serum,Plasma,Urine,Tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (640-660 nm, optimum wavelength: 650 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 3.03 mmol/L |
| Detection Range | 3.64-100 mmol/L |
| Precision | inter-assay CV: 6.5% | intra-assay CV: 0.04 |
| Sample Volume | 30 μL |
| Assay Time | 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human urine | 1 |
| Rat plasma | 1 |
| Porcine serum | 1 |
| 10% Rat heart tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Mouse liver homogenate | 1 |
| 10% Epipremnum aureum leaf tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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