Total Amino Acids (T-AA) Colorimetric Assay Kit

Cat# E-BC-K055-M-96T

Size : 96T

Brand : Elabscience

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Phone : +1 850 650 7790

Detection Principle Copper ions can complex with various amino acids to produce blue-green complex compound, and the depth of color is proportional to the content of total amino acids at a specific wavelength. T-AA content can be calculated with the absorbance at 650 nm.
Synonyms T-AA
Sample Type Serum,Plasma,Urine,Tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (640-660 nm, optimum wavelength: 650 nm)
Research Area Amino Acids And Proteins
Other Reagents Required Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 3.03 mmol/L
Detection Range 3.64-100 mmol/L
Precision inter-assay CV: 6.5% | intra-assay CV: 0.04
Sample Volume 30 μL
Assay Time 40 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
Human urine 1
Rat plasma 1
Porcine serum 1
10% Rat heart tissue homogenate 1
10% Rat liver tissue homogenate 1
10% Mouse liver homogenate 1
10% Epipremnum aureum leaf tissue homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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