Tnf, Recombinant, Rat, aa80-235, His-Tag (Tumor Necrosis Factor, RATTNF, TNF-Alpha, Tnfa)

Tnf also known as Tumor necrosis factor. The Protein acts as a cytokine binds TNF receptors plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response. Recombinant rat Tnf, fused to His-Tag at N-terminal, expressed in E. coli and purified by using conventional chromatography techniques.||Source:|Recombinant protein corresponding to aa80-235 of rat Tnf, fused to His-Tag at N-terminal, expressed in E. coli.||Molecular Weight: |~19.9kD (181aa), Confirmed by MALDI-TOF||Endotoxin: |<1EU/1ug (determined by LAL method)||Amino Acid Sequence:|MGSSHHHHHH SSGLVPRGSH MGSHMLRSSS QNSSDKPVAH VVANHQAEEQ LEWLSQRANA LLANGMDLKD NQLVVPADGL YLIYSQVLFK GQGCPDYVLL THTVSRFAIS YQEKVSLLSA IKSPCPKDTP EGAELKPWYE PMYLGGVFQL EKGDLLSAEV NLPKYLDITE SGQVYFGVIA L||Biological Activity:|Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is less or equal to 0.2ng/ml.||Storage and Stability:|May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.