Thiobarbituric Acid Reactants (TBARS) Colorimetric Assay Kit

Cat# E-BC-K298-M-96T

Size : 96T

Brand : Elabscience

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Phone : +1 850 650 7790

Detection Principle TBARS and TBA can react under high temperature and acid conditions and then form a pink compound, the concentration of which is linearly related to the concentration of TBARS in the sample. The TBARS concentration can be calculated by measuring the OD values at 530-540 nm.
Synonyms TBARS
Sample Type Serum,Plasma,Animal tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (530-540 nm)
Research Area Oxidative Stress
Other Reagents Required Acetic acid, Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.85 μmol/L
Detection Range 2.6-100 μmol/L
Precision inter-assay CV: 7.1% | intra-assay CV: 4.3%
Assay Time 1 h 20 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 1
Rat serum 1
10% Mouse brain tissue homogenization 1
Human serum 1
Rat serum 1
10% Rat liver tissue homogenization 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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