T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone R73, Low Endotoxin
Cat# SM321LE
Size : 500ug
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T Cell Receptor (TCR) alpha/beta Mouse Monoclonal Antibody [Clone ID: R73]
SKU
SM321LE
T Cell Receptor (TCR) alpha/beta mouse monoclonal antibody, clone R73, Low Endotoxin
| Product Data | |
| Clone Name | R73 |
|---|---|
| Application | FC, FN, IHC, IP |
| Recommended Dilution | Functional Assays. Immunoprecipitation. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 100 µl of whole blood or 106 cells in 100 µl. Immunohistochemistry on Frozen Sections: 1/10-1/100. |
| Reactivity | Monkey, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Rat T Blasts and Erythrocytes. Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 mouse myeloma cell line. |
| Specificity | This antibody recognizes a constant determinant on the beta chain of the Alpha/Beta T Cell Receptor, expressed by 97% of peripheral T cells as defined by the OX-52 marker. Clone R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative. This Clone is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes (Colic et al. 2010). |
| Buffer | PBS, pH 7.4 State: Low Endotoxin State: Liquid purified IgG fraction from Tissue Culture Supernatant Stabilizer: None Preservative: None |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Synonyms | TCRA, TCRB, T-Cell Receptor alpha, T-Cell Receptor beta, T-Cell Receptor alpha beta |
| Reference Data | |