Rat Anti-Mouse CD90.2-AF488
Cat# 1750-30
Size : 0.1mg
Brand : Southern Biotech
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Phone : +1 850 650 7790
| Clone | 30-H12 |
|---|---|
| Isotype | Rat (LOU/Ws1/M) IgG2bκ |
| Isotype Control | Rat IgG2b-AF488 (KLH/G2b-1-2) |
| Specificity | Mouse CD90.2 |
| Alternative Names | Thy-1.2 |
| Description | CD90 (Thy-1) is a GPI-anchored molecule and one of the smallest members of the immunoglobulin superfamily consisting of a single V-set domain. The Thy-1.2 alloantigen is expressed on all thymocytes, peripheral T lymphocytes, and some intraepithelial T cells of most mouse strains. It is also expressed in the brain and at varying levels on other non-lymphoid tissues. The 30-H12 monoclonal antibody does not cross react with mouse and rat strains bearing the Thy-1.1 alloantigen (e.g., AKR/J, PL). |
| Immunogen | Mouse thymus or spleen |
| Conjugate | AF488 (Alexa Fluor® 488) |
| Buffer Formulation | Phosphate buffered saline containing < 0.1% sodium azide |
| Clonality | Monoclonal |
| Concentration | 0.5 mg/mL |
| Volume | 0.2 mL |
| Recommended Storage | 2-8°C; Avoid exposure to light |
| Trademark Information | Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries |
| Applications | Flow Cytometry – Quality tested 1,2,9,10 Immunohistochemistry-Frozen Sections – Reported in literature 2,3 Immunocytochemistry – Reported in literature 4 Immunoprecipitation – Reported in literature 1 Depletion – Reported in literature 5,6 Costimulation – Reported in literature 7,8 Apoptosis – Reported in literature 7,8 |
| RRID Number | AB_2795241 |
| Gene ID | 21838 (Mouse) |
| Gene ID Symbol | Thy1 (Mouse) |
| Gene ID Aliases | T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2 |
| UniProt ID | P01831 (Mouse |
| UniProt Name | THY1_MOUSE (Mouse) |
Documentation
Technical Bulletin Safety Datasheet
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- 1. Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90. (Immunogen, FC, IP)
- 2. Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980;152:280-95. (FC, IHC-FS)
- 3. Folgosa L, Zellner HB, El Shikh ME, Conrad DH. Disturbed follicular architecture in B cell A disintegrin and metalloproteinase (ADAM)10 knockouts is mediated by compensatory increases in ADAM17 and TNF-α shedding. J Immunol. 2013;191:5951-8. (IHC-FS)
- 4. Sharma N, Suzuki H, He Q, Sharma RP. Tumor necrosis factor α-mediated activation of c-Jun NH2-terminal kinase as a mechanism for fumonisin B1 induced apoptosis in murine primary hepatocytes. J Biochem Mol Toxicol. 2005;19:359-67. (ICC)
- 5. Sharma N, He Q, Sharma RP. Amelioration of fumonisin B1 hepatotoxicity in mice by depletion of T cells with anti-Thy-1.2. Toxicology. 2006;223:191-201. (Depletion)
- 6. Stevenson MM, Tam MF. Differential induction of helper T cell subsets during blood-stage Plasmodium chabaudi AS infection in resistant and susceptible mice. Clin Exp Immunol. 1993;92:77-83. (Depletion)
- 7. Nakashima I, Zhang Y, Rahman SM, Yoshida T, Isobe K, Ding L, et al. Evidence of synergy between Thy-1 and CD3/TCR complex in signal delivery to murine thymocytes for cell death. J Immunol. 1991;147:1153-62. (Costim, Apop)
- 8. Nakashima I, Pu M, Hamaguchi M, Iwamoto T, Rahman SM, Zhang Y, et al. Pathway of signal delivery to murine thymocytes triggered by co-crosslinking CD3 and Thy-1 for cellular DNA fragmentation and growth inhibition. J Immunol. 1993;151:3511-20. (Costim, Apop)
- 9. Morecki S, Yacovlev E, Gelfand Y, Eizik O, Slavin S. Pretransplant treatment of donors with immunomodulators to control graft-versus-host disease (GVHD) in transplant recipients. Exp Hematol. 2007;35:748-56. (FC)
- 10. Chaimowitz NS, Martin RK, Cichy J, Gibb DR, Patil P, Kang D, et al. A disintegrin and metalloproteinase 10 regulates antibody production and maintenance of lymphoid architecture. J Immunol. 2011;187:5114-22. (FC)