Rat Anti-Mouse CD5-AF488

Cat# 1547-30

Size : 0.1mg

Brand : Southern Biotech

Request more information

Contact local distributor :


Phone : +1 850 650 7790

More Information
Clone 53-7.3
Isotype Rat (LOU/Ws1/M) IgG2aκ
Isotype Control Rat IgG2a-AF488 (KLH/G2a-1-1)
Specificity Mouse CD5
Alternative Names Lyt-1, Ly-1, Lymphocyte antigen 1, Ly-12, Ly-A, T1, Tp67
Description CD5, also known as Lyt-1, is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B-cell antigen CD72. The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells. Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.
Immunogen Spleen cells or thymocyte membranes
Conjugate AF488 (Alexa Fluor® 488)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Monoclonal
Concentration 0.5 mg/mL
Volume 0.2 mL
Recommended Storage 2-8°C; Avoid exposure to light
Trademark Information Alexa Fluor® is a registered trademark of Thermo Fisher Scientific, Inc. or its subsidiaries
Applications Flow Cytometry – Quality tested 1,2
Immunohistochemistry-Frozen Sections – Reported in literature 2
Immunoprecipitation – Reported in literature 1
ELISA – Reported in literature 3
Cytoxicity – Reported in literature 2
Blocking – Reported in literature 4

RRID Number AB_2794868
Gene ID 12507 (Mouse)
Gene ID Symbol Cd5 (Mouse)
Gene ID Aliases Ly-1; Ly-12; Ly-A; Lyt-1
UniProt ID P13379 (Mouse
UniProt Name CD5_MOUSE (Mouse)
Documentation
Technical Bulletin Safety Datasheet

Certificate of Analysis Lookup

Enter the Catalog Number and Lot Number for the Certificate of Analysis you wish to view

  1. 1. Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90. (Immunogen, IP, FC)
  2. 2. Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980;152:280-95. (FC, IHC-FS)
  3. 3. Luo W, van de Velde H, von Hoegen I, Parnes JR, Thielemans K. Ly-1 (CD5), a membrane glycoprotein of mouse T lymphocytes and a subset of B cells, is a natural ligand of the B cell surface protein Lyb-2 (CD72). J Immunol. 1992;148:1630-4. (ELISA)
  4. 4. Bikah G, Lynd FM, Aruffo AA, Ledbetter JA, Bondada S. A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5. Int Immunol. 1998;10:1185-96. (Block)