E-OSEL-C0004-48T | QuicKey Pro Canine Pg (Progesterone) ELISA Kit

Product Summary

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Sensitivity	6.58 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	50 μL
Total Assay Time	1 h 30 min
Reacitivity	Canine
Specificity	This kit recognizes Canine Pg in samples.No significant cross-reactivity or interference between Canine Pg and analogues was observed
Recovery	80%-120%
Sample Type	serum, plasma
Detection Method	Colorimetric method, ELISA, Competitive
Assay Type	Competitive-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	6 months

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Canine Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Canine Pg are added to the micro ELISA plate wells. Canine Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Canine Pg in the samples is then determined by comparing the OD of the samples to the standard curve.

Background

Canine progesterone无progesterone (Pg) refers to a human-like progesterone secreted in animals (especially dogs), which is mainly produced by the ovaries to support and safeguard early growth and development stages. We can draw the following conclusions: Progesterone levels and optimal time to breed: Studies have shown that serum progesterone levels increase during estrus and rise to a peak a few days before ovulation. This means that by monitoring changes in progesterone levels, the best time to breed can be found. The importance of progesterone detection: The rise of progesterone levels happens to start after the peak of LH, so by tracking the change in progesterone levels, it can be judged when the female dog eggs are discharged and mature in large numbers, effectively improving the pregnancy rate. Progesterone levels and reproductive capacity: The effects of meloxicam administration during ovulation on luteal function in domestic dogs have been observed in a study in which a 2 year old female German Shepherd, whose progesterone levels indicated ovulation (5.47 ng无mL), received a single subcutaneous injection. This suggests a correlation between progesterone levels and reproductive capacity. False pregnancy: Even if not pregnant, dogs will have luteoid function for about 2 months. This means that even if the dog is not pregnant, the mammary glands will be enlarged to some extent under the action of the hormone (progesterone). In summary, canine progesterone无progesterone (Pg) is mainly concerned with determining the best time to breed by monitoring changes in progesterone levels and, in this way, improving reproductive success. At the same time, it is also necessary to pay attention to the situation of false pregnancy, even if there is no pregnancy, the dog's mammary gland will have a certain degree of enlargement under the action of hormones.

Research Area

Signal Transduction

QuicKey Pro Canine Pg (Progesterone) ELISA Kit

Cat# E-OSEL-C0004-48T

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Brand : Elabscience

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