Pyruvate Kinase (PK) Activity Assay Kit
Cat# E-BC-K611-M-48T
Size : 48T
Brand : Elabscience
Contact local distributor :
Phone : +1 850 650 7790
| Detection Principle | In the presence of adenosine diphosphate (ADP), pyruvate kinase (PK) catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, Lactate dehydrogenase catalyzes the reaction of pyruvic acid with NADH to produce lactic acid and NAD+. The activity of pyruvate kinase can be calculated by detection the absorbance changes at 340 nm. |
| Synonyms | PK |
| Sample Type | Serum,Plasma,Tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (330-350 nm,optimum wavelength: 340 nm) |
| Research Area | Glycolysis And Lipid Metabolism , Tricarboxylic Acid（TCA）Cycle , Energy Metabolism |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.08 U/L |
| Detection Range | 0.08-6.79 U/L |
| Precision | inter-assay CV: 0.07 | intra-assay CV: 0.05 |
| Sample Volume | 10 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat kidney tissue homogenate | 4-6 |
| 10% Rat lung tissue homogenate | 4-6 |
| 10% Rat liver tissue homogenate | 4-6 |
| 10% Rat brain tissue homogenate | 4-6 |
| 10% Mouse liver tissue homogenate | 4-6 |
| 10% Mouse heart tissue homogenate | 4-6 |
| Rat serum | 1 |
| Rat plasma | 1 |
| Mouse serum | 1 |
| Mouse plasma | 1 |
| Human serum | 1 |
| Dog plasma | 1 |
| HL-60 cell | 1 |
| 293T cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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