Reagents and instruments for immunology, cell biology and molecular biology.
 
> PID1 Polyclonal Antibody

PID1 Polyclonal Antibody

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Cat# E-AB-90611-60

Size : 60uL

Brand : Elabscience

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Phone : +1 850 650 7790

Verified Samples Verified Samples in WB: various cell lines
Verified Samples in IP: Mouse liver
Dilution WB 1:500-1:2000,  IP 1:50-1:200
Isotype IgG
Host Rabbit
Reactivity Human,  Mouse,  Rat
Applications WB,  IP
Clonality Polyclonal
Immunogen Recombinant fusion protein of human PID1
Abbre PID1
Synonyms HMFN2073,  NYGGF4,  P-CLI1,  PCLI1,  PID1
Swissprot
Calculated MW 19 kDa/24 kDa/28 kDa
Observed MW 27 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization Cytoplasm
Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification Method Affinity purification
Research Areas Tags,  Cell Markers,  Cell Biology,  Metabolism
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background Increases proliferation of preadipocytes without affecting adipocytic differentiation.
Cat.No. Product Name Sizes
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E-AB-1043 Streptavidin(peroxidase/HRP conjugated) 500μL , 120μL , 60μL
E-AB-1194 HRP-Goat Anti-Rabbit IgG(H+L) preadsorbed 500μL , 120μL , 1mL
E-IR-R304A Western Blot Detection Kit 50Assays
E-IR-R304B High Accuracy and Absorbability Western Blot Detection Kit 50Assays
Other Clones

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Other Formats

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Unconjugated

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