Pembrolizumab [1374853-91-4]

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Cat# HY-P9902-1mg

Size : 1mg

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Brand : MedChemExpress

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Description

Pembrolizumab (MK-3475) is a humanized IgG4 antibody inhibiting the programmed cell death 1 (PD-1) receptor, used in cancer immunotherapy.

Isotype

Human IgG4 kappa

Recommend Isotype Controls

Human IgG4 (S228P) kappa, Isotype Control

Species

Humanized

IC₅₀ & Target

IC50: 0.6 nM (PD-1)

In Vitro

Pembrolizumab (MK-3475) increases the secretion of cytokines IFN-γ, TNF-α and the apoptotic cell death of A549 cells^[2].
Pembrolizumab improves the αROR1-CAR T-mediated cytotoxicity, and reduces tumorigenesis in the co-cultured αROR1-CAR T and A549 cells by blocking PD-1::PD-L1 interaction in αROR1-CAR T cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay^[2]

Cell Line:	αROR1-CAR T and A549 cells
Concentration:	10 μg/mL
Incubation Time:	Adding Pembrolizumab after A549-Red-Fluc cells preconditioned in elevated pressure (+ 100 mmHg) for 7 days.
Result:	Enhanced αROR1-CAR T killing of A549 lung cancer cells under elevated pressure.

Apoptosis Analysis^[2]

Cell Line:	A549 cells
Concentration:	10 μg/mL
Incubation Time:
Result:	Increased the apoptotic cell death in A549 cells, and improve the αROR1-CAR T-mediated cytotoxicity.

In Vivo

Pembrolizumab inhibits tumor size in A549 xenograft mice pretreated by pressure^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six-week-old male BALB/c athymic nude mice^[2]
Dosage:	5 mg/kg
Administration:	Pembrolizumab (5 mg/kg; Intravenous injection; Once)
Result:	Reduced tumorigenesis in αROR1-CAR T cells and co-cultured with A549 cells.

Clinical Trial

CAS No.

1374853-91-4

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[Pembrolizumab]

Shipping

Shipping with dry ice.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Format

Human IgG4 kappa

Biological Activity

Flow cytometry analysis of PD-1 overexpression HEK293 cells labelling PD-1 with Pembrolizumab at 67nM (Red). Goat anti human IgG Fc(Alexa Fluor488) was used as the secondary antibody at 1/400 dilution. Isotype control - human IgG (Blue). Unlabeled control - Unlabelled cells (Gray).
Loaded Pembrolizumab on AHC2 biosensor, can bind PD-1 Protein, Human (143a.a, HEK293, His, HY-P7396) with an affinity constant of 1.898E-09 M as determined in BLI assay.
Immobilized PD-1 Protein, Human can bind Pembrolizumab. The EC₅₀ for this effect is 1.8 ng/mL.