One-step HIV-1 p24 Protein ELISA Kit 1 x 96
Cat# EA290002
Size : 1x96wells
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One-step HIV-1 p24 Protein ELISA Kit 1 x 96
| Product Data | |
| ACCN | ACI05538 |
|---|---|
| Assay Length | 1.5 hours incubations; 10 minutes washing and analyzing samples |
| Assay Platform | 96-well plate |
| Assay Type | Sandwich |
| Assay Type | Sandwich |
| Background | The gag protein p24 (MW 24kD) is a major structural protein of the HIV capsid, which is required for virus particle assembly . There are approximately 2000 p24 molecules per virus particle, or at a molecule weight of 24 kDa, about 104 virus particles per picogram of p24. The onset of symptoms of AIDS correlates with an increased level of virus and p24 in the blood. HIV-1 p24 measurement is commonly used as an indicator of HIV-1 infection and viral load. In gene therapy, HIV-derived lentiviral vectors are commonly used to deliver transgenes to mammalian cells. Quantitative detection of the HIV-1 p24 has become a standard method for measuring lentiviral transduction efficiency. |
| Components |
|
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Curve Range | 125-2000 pg/mL |
| Format | 8x12 divisible strips |
| incubation_time | 20 mins |
| Note | Review complete instructions before performing the test. Strips of ELISA plate are removable. Remove unused strips and store as described under "Storage and Stability". Before testing begins, the user should inspect ELISA strip holders and ensure all strips are secure. A white stabilizer residue is normally observed on the bottom of unused wells. |
| Reactivity | Virus |
| Reactivity | Virus |
| Reactivity | Virus |
| Sample Type | Cell cuture supernatant, lentiviral sample |
| Sample Volume | 1µl of lentiviral sample or 90µl diluted sample |
| Sensitivity | 4.18pg/mL |
| Signal | Colorimetric |
| Species | HIV-1 |
| Specificity | This kit is used for the detection of HIV-1 p24 protein |
| New | No |
| Description | One-step HIV-1 p24 Protein ELISA Kit 1 x 96 |