NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker) (ENO2/1375), 0.2mg/mL
Cat# BNUB1375-100
Size : 100uL
Brand : Biotium
Contact local distributor :
Phone : +1 850 650 7790
| Antibody number | #1375 |
|---|---|
| Antibody reactivity (target) | NSE-Gamma |
| Antibody type | Primary |
| Host species | Mouse |
| Clonality | Monoclonal |
| Clone | ENO2/1375 |
| Isotype | IgG2b |
| Molecular weight | ~50 kDa |
| Synonyms | 2-phospho-D-glycerate hydrolyase; ENO2; ENOG; Enolase 2 gamma neuronal; Enolase2; Gamma-enolase; Neural enolase; Neuron specific gamma enolase; Neuron-specific enolase; NSE |
| Human gene symbol | ENO2 |
| Entrez gene ID | 2026 |
| SwissProt | P09104 |
| Unigene | 511915 |
| Immunogen | A synthetic peptide corresponding to aa416-433 of human NSE gamma (exact sequence is proprietary) |
| Antibody target cellular localization | Cytoplasmic |
| Verified antibody applications | IHC (FFPE) (verified), WB (verified) |
| Species reactivity | Human, Mouse, Rat |
| Antibody application notes | Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Immunofluorescence: 1-2 ug/mL, Immunohistology (formalin) 0.1-0.2 ug/mL, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min, Flow Cytometry 0.5-1 ug/million cells/0.1 mL, Western blotting 0.5-1 ug/mL, Optimal dilution for a specific application should be determined by user |
| Positive control | HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma. |
| Shipping condition | Room temperature |
| Storage Conditions | Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C |
| Shelf life | Guaranteed for at least 24 months from date of receipt when stored as recommended |
| Regulatory status | For research use only (RUO) |
| Antibody/conjugate formulation | Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide |
| Antibody research areas | Cancer, Metabolism, Neuroscience |
| Product origin | Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot. |
| Cell/tissue expression | Neurons |
| Tumor expression | Neuroendocrine cancer |
Product Description
This antibody recognizes a protein of about 50 kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the
CF® Dye Brochure
for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.info@biovalley.fr
to inquire about stock status and lead times before placing your order.Catalog number key for antibody number 1375, Anti-NSE-Gamma (ENO2/1375)
| Antibody # prefix | Conjugation | Ex/Em (nm) | Laser line | Detection channel | Dye Features |
|---|---|---|---|---|---|
| BNC04 | CF®405S | 404/431 | 405 | DAPI (microscopy), AF405 | CF®405S Features |
| BNC88 | CF®488A | 490/515 | 488 | GFP, FITC | CF®488A Features |
| BNC68 | CF®568 | 562/583 | 532, 561 | RFP, TRITC | CF®568 Features |
| BNC94 | CF®594 | 593/614 | 561 | Texas Red® | CF®594 Features |
| BNC40 | CF®640R | 642/662 | 633-640 | Cy®5 | CF®640R Features |
| BNC47 | CF®647 | 650/665 | 633-640 | Cy®5 | CF®647 Features |
| BNC74 | CF®740 | 742/767 | 633-685 | 775/50 | CF®740 Features |
| BNCB | Biotin | N/A | N/A | N/A | |
| BNUB | Purified | N/A | N/A | N/A | |
| BNUM | Purified, BSA-free | N/A | N/A | N/A |