ELISA: The antibody (approx. 1:100,000 - 1:400,000 diluted) can detect the serine 337 phosphorylated NF-κB p105/p50 peptide coated on ELISA plate at 100 ng/well.
Reactivity
Human, Mouse
Antibody Host
Rabbit
Clonality
Polyclonal
Immunogen
A synthetic peptide derived from Serine337 phosphorylated NF-κB p105/p50, conjugated with KLH
Specificity
This antibody is reactive to the synthetic NF-κB p105/p50 peptide (QLRRKSpDLETSEP) on Mouse, human and other species with consensus serine 337 phosphorylated NF- κB p105/p50 peptide.
Restore with double distilled water to adjust the final concentration to 1.00 mg/ml.
Purification
Protein G affinity
Conjugation
Unconjugated
Storage
Store lyophilized at 2-8°C for 6 month or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
NF-κB (nuclear factor kappa-light-chainenhancer of activated B cells) is a transcription factor that controls the transcription of many genes that are associated with immuno-response, antiapoptosis, neuroplasticity and memory. There are five NF-κB proteins in mammals: RelA (NF-κB-p65), RelB, c-Rel, NF-κB (NF-κB p105), and NF-κB2 (NF-κB p100). They form a variety of homodimers and heterodimers, each of which activates its own distinctive set of genes. P65 has been shown to be involved with DNA binding. The phosphorylation of p65 at Ser468 is inducible by TNF. Ser468 phosphorylation mediates p65 ubiquitination and the termination of related gene expression.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
