Reagents and instruments for immunology, cell biology and molecular biology.
 
> Mouse Ig Kappa Chain Antibody : TRITC

Mouse Ig Kappa Chain Antibody : TRITC

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Cat# OASB01545

Size : 1.0mg

Brand : Aviva Systems Biology

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Datasheets/ManualsPrintable datasheet for OASB01545
Product Info
Tested Species ReactivityMouse
ClonalityPolyclonal
IsotypeIgG
HostGoat
ConjugationTRITC: Tetramethylrhodamine
ApplicationFLISA, IHC-F, IHC-P, ICC
Additional InformationDescription: Pooled antisera from goats hyperimmunized with mouse kappa light chains; purified by affinity chromatography on mouse kappa light chains covalently linked to agarose; reacts with mouse kappa light chains; cross-adsorbed against mouse lambda light chains for minimal reactivity
Reconstitution and StorageStore at 2-8C
ImmunogenMouse kappa light chains
PurificationAffinity chromatography on pooled mouse Igs with- - light chains covalently linkedto agarose.
Concentration1.0 mg/mL
SpecificityKappa
CharacterizationTo insure lot- to- lot consistency, each batch of product is tested by ELISA, FLISA, and/or flow cytometry forconformance with characteristics of a standard reference reagent.
WarningReagents contain sodium azide which is very toxic if ingested or inhaled. Avoid contact with skin, eyes, orclothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.
DilutionELISA:
FLISA:
AP conjugate
HRP conjugate
BIOT conjugate
TRITC and TXRD conjugates
1:2,000-1:4,000
1:4,000-1:8,000
1:5,000-1:20,000
1:100-1:400
Immunofluorescence:
Purified antibody
FITC conjugates
R-PE conjugate
<= 1 ug/106 cells
<= 1 ug/106 cells
<= 0.1 ug/106 cells
Application InfoEnzyme-Linked-Immunosorbent-Assay (ELISA), Fluorescent-Linked-Immunosorbent-Assay (FLISA), Immunoblotting, Immunohistochemistry
Other Applications DataSince applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need.
Storage- The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM boratebuffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C
- The fluorescein (FITC), rhodamine (TRITC), and Texas Red (TXRD) conjugates are supplied as 0.5 mgin 1.0 mL PBS/NaN3. Store at 2- 8 C
- The alkaline phosphatase (AP) conjugate is supplied as 1.0 mL of stock solution in 50mM Tris/1mMMgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative. Store at 2- 8 C or long- term at - 20 C
- The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50%PBS, pH 7.4. No preservative added. Store at 2- 8 C or long- term at - 20 C
- The biotin (BIOT) conjugate is supplied as 1.0 mg in 2.0 mL PBS/NaN3. Store at 2- 8 C
- The R- phycoerythrin (R- PE) conjugate is supplied as 0.25 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze!
- Protect fluorochrome- conjugated forms from light. Reagents are stable for the period shown on the label ifstored as directed.
Cross AbsorptionPooled mouse paraproteins with  light chains.
Gene SymbolIGK
Gene Full NameIg kappa chain C region
Alias Symbolsk, kappa
NCBI Gene Id243469
Description of TargetSummary:Immunoglobulins recognize foreign antigens and initiate immune responses such as phagocytosis and the complement system. Each immunoglobulin molecule consists of two identical heavy chains and two identical light chains. There are two classes of light chains, kappa and lambda. This region represents the germline organization of the kappa light chain locus from the C57BL/6J inbred mouse strain. The locus includes V (variable), J (joining), and C (constant) segments. During B cell development, a recombination event at the DNA level joins a single V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase, and by somatic hypermutation, which occurs during B cell maturation in the spleen and lymph nodes. Several V segments in this cluster are incapable of encoding a protein and are considered pseudogenes.
Uniprot IDP01837

  1. What is the species homology for "Goat Anti-Mouse Kappa Antibody - TRITC Conjugated (OASB01545)"?

    The tested species reactivity for this item is "Mouse". This antibody is predicted to have homology to "".

  2. How long will it take to receive "Goat Anti-Mouse Kappa Antibody - TRITC Conjugated (OASB01545)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "Goat Anti-Mouse Kappa Antibody - TRITC Conjugated (OASB01545)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "Goat Anti-Mouse Kappa Antibody - TRITC Conjugated (OASB01545)"?

    This target may also be called "k, kappa" in publications.

  5. What is the shipping cost for "Goat Anti-Mouse Kappa Antibody - TRITC Conjugated (OASB01545)"?

    The shipping cost for this item is within the US. Please contact us for specific shipping for international orders.

  6. What is the guarantee for "Goat Anti-Mouse Kappa Antibody - TRITC Conjugated (OASB01545)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "Goat Anti-Mouse Kappa Antibody - TRITC Conjugated (OASB01545)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "Goat Anti-Mouse Kappa Antibody - TRITC Conjugated (OASB01545)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "IGK"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "IGK"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "IGK"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "IGK"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "IGK"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "IGK"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.