Mix-n-Stain™ CF®350 Antibody Labeling Kit, 1x(50-) labeling
Cat# 92230
Size : 1kit
Brand : Biotium
Contact local distributor :
Phone : +1 850 650 7790
Mix-n-Stain™ CF® Dye Antibody Labeling Kits
Label your IgG antibody with one of our superior CF® Dyes in as little as 15 minutes without a purification step. Labeling tolerates many common buffer components including BSA and ascites.
Technology
CF® Dyes
CF® Dyes for Multi-Color Super-Resolution Microscopy
Antibody & Protein Labeling Kits
Protocols
Supporting Documents
Product Description
Mix-n-Stain™ CF® Dye Antibody Labeling Kits allow you to label ≤5 ug up to 100 ug of your IgG antibody in as little as 15 minutes, with minimal hands-on time and no purification.
Note: This kit is for labeling IgG antibodies only. For labeling IgM antibodies, please see our Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits.
Features
- Label 5-20 ug, 20-50 ug, or 50-100 ug Ab
- Choice of over 30 CF® Dye colors
- Less than 30 seconds of hands-on time
- 15 minutes total reaction time
- No purification, 100% recovery
- Compatible with BSA, gelatin, ascites
Kit components
- Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed)
- 10X Mix-n-Stain™ Reaction Buffer
- Lyophilized Reactive CF® Dye
- Mix-n-Stain™ Storage Buffer
Unrivaled Convenience
Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. Because CF® Dyes are highly water soluble, the presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.
Superior CF® Dyes
These kits feature a choice of 31 of Biotium’s next-generation CF® Dyes, which were designed for advantages in brightness and photostability compared to other commercially available fluorescent dyes. Learn more about CF® Dyes.
Choose the Right Labeling Kit for Your Antibody
Mix-n-Stain™ Antibody Labeling Kits are very simple to use (see the workflow overview above). But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. Download the updated and expanded Product Protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see our Kit Compatibility and Protocol Selection Flowchart.
See the product table below for a quick reference of the maximum excitation and emission of each CF® Dye, or see our CF® Dye Brochure for more detailed dye information.
Other Labeling Kits
We now offer Mix-n-Stain™ Maxi Antibody Labeling Kits for labeling 1 mg of IgG antibody with CF® Dyes, as well as Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits and Mix-n-Stain™ Nanobody Labeling Kits. Mix-n-Stain™ kits also are available for labeling antibodies with biotin, DNP, or DIG. See our full selection of Mix-n-Stain™ Antibody Labeling Kits.
Mix-n-Stain™ CF® Dye Antibody Labeling Kits
| Conjugation | Ex/Em | Size | Catalog No. | Dye Features |
|---|---|---|---|---|
| CF®350 | 347/448 nm | 5-20 ug | 92270 | CF®350 Features |
| 20-50 ug | 92250 | |||
| 50-100 ug | 92230 | |||
| CF®405S | 404/431 nm | 5-20 ug | 92271 | CF®405S Features |
| 20-50 ug | 92251 | |||
| 50-100 ug | 92231 | |||
| CF®405M | 408/452 nm | 5-20 ug | 92272 | CF®405M Features |
| 20-50 ug | 92252 | |||
| 50-100 ug | 92232 | |||
| CF®405L | 395/545 nm | 5-20 ug | 92303 | CF®405L Features |
| 20-50 ug | 92304 | |||
| 50-100 ug | 92305 | |||
| 3x(5-20 ug) | 92445 | |||
| CF®430 | 426/498 nm | 5-20 ug | 92316 | CF®430 Features |
| 20-50 ug | 92317 | |||
| 50-100 ug | 92318 | |||
| CF®440 | 440/515 nm | 5-20 ug | 92319 | CF®440 Features |
| 20-50 ug | 92320 | |||
| 50-100 ug | 92321 | |||
| CF®450 | 450/538 nm | 5-20 ug | 92322 | CF®450 Features |
| 20-50 ug | 92323 | |||
| 50-100 ug | 92324 | |||
| CF®488A | 490/515 nm | 5-20 ug | 92273 | CF®488A Features |
| 20-50 ug | 92253 | |||
| 50-100 ug | 92233 | |||
| 3x(5-20 ug) | 92446 | |||
| CF®514 | 516/548 nm | 5-20 ug | 92331 | CF®514 Features |
| 20-50 ug | 92332 | |||
| 50-100 ug | 92333 | |||
| CF®532 | 527/558 nm | 5-20 ug | 92289 | CF®532 Features |
| 20-50 ug | 92290 | |||
| 50-100 ug | 92291 | |||
| CF®543 | 541/560 nm | 5-20 ug | 92287 | CF®543 Features |
| 20-50 ug | 92267 | |||
| 50-100 ug | 92247 | |||
| CF®555 | 555/565 nm | 5-20 ug | 92274 | CF®555 Features |
| 20-50 ug | 92254 | |||
| 50-100 ug | 92234 | |||
| CF®568 | 562/583 nm | 5-20 ug | 92275 | CF®568 Features |
| 20-50 ug | 92255 | |||
| 50-100 ug | 92235 | |||
| 3x(5-20 ug) | 92447 | |||
| CF®570 | 568/591 nm | 5-20 ug | 92334 | CF®570 Features |
| 20-50 ug | 92335 | |||
| 50-100 ug | 92336 | |||
| CF®583 | 583/606 nm | 5-20 ug | 92337 | CF®583 Features |
| 20-50 ug | 92338 | |||
| 50-100 ug | 92339 | |||
| CF®583R | 586/609 nm | 5-20 ug | 92455 | CF®583R Features |
| 20-50 ug | 92456 | |||
| 50-100 ug | 92457 | |||
| CF®594 | 593/614 nm | 5-20 ug | 92276 | CF®594 Features |
| 20-50 ug | 92256 | |||
| 50-100 ug | 92236 | |||
| 3x(5-20 ug) | 92448 | |||
| CF®597R | 597/619 nm | 5-20 ug | 92461 | CF®597R Features |
| 20-50 ug | 92462 | |||
| 50-100 ug | 92463 | |||
| CF®633 | 630/650 nm | 5-20 ug | 92277 | CF®633 Features |
| 20-50 ug | 92257 | |||
| 50-100 ug | 92237 | |||
| CF®640R | 642/662 nm | 5-20 ug | 92278 | CF®640R Features |
| 20-50 ug | 92258 | |||
| 50-100 ug | 92245 | |||
| CF®647 | 650/665 nm | 5-20 ug | 92279 | CF®647 Features |
| 20-50 ug | 92259 | |||
| 50-100 ug | 92238 | |||
| 3x(5-20 ug) | 92449 | |||
| CF®660R | 663/682 nm | 5-20 ug | 92281 | CF®660R Features |
| 20-50 ug | 92261 | |||
| 50-100 ug | 92243 | |||
| CF®660C | 667/685 nm | 5-20 ug | 92280 | CF®660C Features |
| 20-50 ug | 92260 | |||
| 50-100 ug | 92239 | |||
| CF®680 | 681/698 nm | 5-20 ug | 92282 | CF®680 Features |
| 20-50 ug | 92262 | |||
| 50-100 ug | 92240 | |||
| CF®680R | 680/701 nm | 5-20 ug | 92283 | CF®680R Features |
| 20-50 ug | 92263 | |||
| 50-100 ug | 92246 | |||
| CF®700 | 695/720 nm | 5-20 ug | 92425 | CF®700 Features |
| 20-50 ug | 92426 | |||
| 50-100 ug | 92427 | |||
| CF®750 | 755/777 nm | 5-20 ug | 92284 | CF®750 Features |
| 20-50 ug | 92264 | |||
| 50-100 ug | 92241 | |||
| CF®770 | 770/797 nm | 5-20 ug | 92285 | CF®770 Features |
| 20-50 ug | 92265 | |||
| 50-100 ug | 92242 | |||
| CF®790 | 784/806 nm | 5-20 ug | 92288 | CF®790 Features |
| 20-50 ug | 92268 | |||
| 50-100 ug | 92248 | |||
| CF®800 | 797/816 nm | 5-20 ug | 92428 | CF®800 Features |
| 20-50 ug | 92429 | |||
| 50-100 ug | 92430 | |||
| CF®820 | 822/835 nm | 5-20 ug | 92431 | CF®820 Features |
| 20-50 ug | 92432 | |||
| 50-100 ug | 92433 |
Antibody & Protein Labeling Kits
| Product Name | Label Options | Labeling Scale | Labeling Time | Features |
|---|---|---|---|---|
| Mix-n-Stain™ CF® Dye Antibody Labeling Kits | CF® Dyes | ≤5-20 ug IgG 20-50 ug IgG 50-100 ug IgG | ~ 15 min. | • Rapid, simple labeling • No purification • Tolerates BSA & other additives • Dyes for super-resolution, spectral flow, & NIR detection |
| Mix-n-Stain™ FITC Antibody Labeling Kits | FITC | |||
| Mix-n-Stain™ Cyanine Dye Antibody Labeling Kits | Cyanine 555 Cyanine 647 | |||
| Mix-n-Stain™ Biotin Antibody Labeling Kits | Biotin | |||
| Mix-n-Stain™ Digoxigenin Antibody Labeling Kits | Digoxygenin (DIG) | |||
| Mix-n-Stain™ DNP Antibody Labeling Kits | DNP | |||
| Mix-n-Stain™ Maxi Antibody Labeling Kits | CF® Dyes Cyanine Dyes | 1 mg IgG | ~ 30 min. | |
| Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits | CF® Dyes FITC | 25 ug IgM 100 ug IgM | 15-30 min. | • Rapid, simple labeling • No purification |
| Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits | CF® Dyes | 50 ug IgG | ~ 30 min. | • Optimized to provide low 1-2.5 DOL • No purification |
| Mix-n-Stain™ Nanobody Labeling Kits | CF® Dyes | 5-20 ug Nanobody® 20-50 ug Nanobody® | ~ 30 min. | • Optimized for Nanobodies® • Tolerates BSA & other additives |
| Mix-n-Stain™ Small Ligand Labeling Kits | CF® Dyes | 0.1 umol small ligand | ~ 30 min. | • Label SNAP®, CLIP™, HaloTag® & other small ligands • Dye options for surface or intracellular targets |
| VivoBrite™ Rapid Antibody Labeling Kits for Small Animal In Vivo Imaging | Near-IR CF® Dyes | 3 x 1 mg IgG | ~ 2 hours | • Dyes, buffers, & spin vials for labeling + purification • Sterile syringes, filters and storage vials for in vivo use |
| CF® Dye & Biotin SE Protein Labeling Kits | CF® Dyes Biotin | 3 x 1 mg protein | ~ 2 hours | • Dyes, buffers, & spin vials for labeling + purification |
Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific.
References
Download a list of CF® Dye and Mix-n-Stain™ references.
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