MHC Class I (HRP)

Recognizes the ovine homologue of the human MHC Class I, a monomorphic determinant expressed on the heavy chain of sheep MHC Class I, (OLA Class I).||The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In sheep, this is often referred to as the ovine leukocyte antigen (OLA) region. Ovine MHC Class I functions in the recognition and presentation of foreign antigens to T-cells.||Ovine MHC Class I is a membrane glycoprotein with a molecular weight of ~44kD, expressed on the cell surface of all peripheral blood leucocytes.||Has been in used in studies on a number of domestic animal diseases, in particular Maedi Visna virus infection, a disease of significant inportance in commercial sheep flocks (Lee et al. 1996, Ryan et. al. 2000 and Wu et. al. 2008). Has been cited as recognising MHC class I in some other species and has been used in a study of Feline herpes virus infection (Montagnaro et. al. 2009).||Applications: |Suitable for use in Flow Cytometry (Not Tested), Western Blot, Immunoprecipitation, and Immunohistochemistry. Other applications not tested.||Recommended Dilution:|Flow Cytometry (Not Tested): 1:25-1:200|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. |For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. ||Note: Applications are based on unconjugated antibody.