Western Blot (Cell lysate)
Western blot analysis using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K-562 (6), Jurkat (7), HeLa (8) and MCF-7 (9) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) against human MCM2 (aa: 16-232) recombinant protein. (Expected MW is 50.4 kDa)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer (A) and human lung cancer (B) tissues using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) with DAB staining.
Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805).
Flow Cytometry
Flow cytometric analysis of HeLa cells using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) (blue) and negative control (red).
- Specifications
Product Description
Mouse monoclonal antibody raised against partial recombinant MCM2.
Immunogen
Recombinant protein corresponding to human MCM2.
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat
Form
Liquid
Isotype
IgG1
Recommend Usage
ELISA (1:10000)
Western Blot (1:500-1:2000)
Immunohistochemistry (1:200-1:1000)
Flow cytometry (1:200-1:400)
The optimal working dilution should be determined by the end user.Storage Buffer
In ascites (0.03% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Western Blot (Cell lysate)
Western blot analysis using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) against PC-12 (1), Cos7 (2), NIH/3T3 (3), HepG2 (4), HEK293 (5), K-562 (6), Jurkat (7), HeLa (8) and MCF-7 (9) cell lysate.Western Blot (Recombinant protein)
Western blot analysis using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) against human MCM2 (aa: 16-232) recombinant protein. (Expected MW is 50.4 kDa)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemical analysis of paraffin-embedded human colon cancer (A) and human lung cancer (B) tissues using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) with DAB staining.Enzyme-linked Immunoabsorbent Assay
ELISA measurement of MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805).Flow Cytometry
Flow cytometric analysis of HeLa cells using MCM2 monoclonal antibody, clone 2B3 (Cat # MAB10805) (blue) and negative control (red). - Gene Info — MCM2
Entrez GeneID
4171Gene Name
MCM2
Gene Alias
BM28, CCNL1, CDCL1, D3S3194, KIAA0030, MGC10606, MITOTIN, cdc19
Gene Description
minichromosome maintenance complex component 2
Omim ID
116945Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. [provided by RefSeq
Other Designations
DNA replication licensing factor MCM2|MCM2 minichromosome maintenance deficient 2, mitotin|cell devision cycle-like 1|cyclin-like 1|minichromosome maintenance deficient 2 (mitotin)|nuclear protein BM28
- Interactomes
- Pathways
- Diseases