MBP Polyclonal Antibody
Cat# E-AB-70265-120
Size : 120uL
Brand : Elabscience
Contact local distributor :
Phone : +1 850 650 7790
| Verified Samples | Verified Samples in WB: Mouse brain, Mouse hippocampus, Mouse cerebellum, Rat brain, Rat hippocampus, Rat cerebellum Verified Samples in IHC: Mouse brain, Rat brain |
| Dilution | WB 1:500-1:1000, IHC 1:200-1:600 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | KLH conjugated Synthetic peptide corresponding to Mouse Myelin Basic Protein |
| Abbre | MBP |
| Synonyms | GDB, Golli MBP, HMBPR, HUGO, Hemopoietic MBP, MBP, MBP_CAVPO, MGC99675, MLD, Myelin A1 Protein, Myelin A1 protein, Myelin Deficient, Myelin basic protein, Myelin membrane encephalitogenic protein, OTTHUMP00000, OTTHUMP00000163776, basic, myelin basic protein |
| Swissprot | |
| Calculated MW | 17-22 kDa |
| Observed MW | 17-22 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Myelin membrane. Cytoplasmic side of myelin. |
| Concentration | 300 μg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Neuroscience, Tags and Cell Markers |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | MBP belongs to the myelin basic protein family. The classic group of MBP isoforms (isoform 4-isoform 14) are the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. MBP has six isoforms. Catalog#10458-1-AP is capable of recognizing multiple isoforms of MBP. |
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