KIF4A Polyclonal Antibody

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Cat# E-AB-63295-60

Size : 60uL

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Phone : +1 850 650 7790

Brand : Elabscience

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Phone : +1 850 650 7790

Verified Samples	Verified Samples in WB: U87-MG, L-O2, 293T, SW480 Verified Samples in IHC: Rat heart, Human stomach, Mouse kidney Verified Samples in IF: HeLa
Dilution	WB 1:1000-1:3000, IHC 1:50-1:200, IF 1:100-1:200
Isotype	IgG
Host	Rabbit
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF
Clonality	Polyclonal
Immunogen	Recombinant fusion protein of human KIF4A (NP_036442.3).
Abbre	KIF4A
Synonyms	KIF4, KIF4A, KIF4G1, MRX100
Swissprot	O95239
Calculated MW	128 kDa/139 kDa
Observed MW	140 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Nucleus matrix. Cytoplasm>cytoskeleton>spindle. Midbody. Chromosome. Not present in the nucleolus. In early mitosis, associated with the mitotic spindle, in anaphase, localized to the spindle midzone and, in telophase and cytokinesis, to the midbody. In late cytokinesis, found in the center of the midbody. Associated with chromosomes at all stages of mitosis.
Concentration	1 mg/mL
Buffer	Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol.
Purification Method	Affinity purification
Research Areas	Cancer, Cell Biology, Epigenetics and Nuclear Signaling, Signal Transduction
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended.
background	This gene encodes a member of the kinesin 4 subfamily of kinesin related proteins. The encoded protein is an ATP dependent microtubule-based motor protein that is involved in the intracellular transport of membranous organelles. This protein also associates with condensed chromosome arms and may be involved in maintaining chromosome integrity during mitosis. This protein may also be involved in the organization of the central spindle prior to cytokinesis. A pseudogene of this gene is found on chromosome X.

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