Reagents and instruments for immunology, cell biology and molecular biology.
 
> Isotype Control, Mouse IgG1 Kappa (IGG1/1331), APC Conjugate, 0.1 mg/mL

Isotype Control, Mouse IgG1 Kappa (IGG1/1331), APC Conjugate, 0.1 mg/mL

Print Print
Please log in

Cat# BNCA1331-250

Size : 250uL

Brand : Biotium

Contact local distributor :


Phone : +1 850 650 7790

Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331)

Non-reactive antibody to use as a negative control for mouse IgG1 primary antibodies. Available with CF® Dyes and other labels.

Product Attributes

Antibody number

#1331
Antibody reactivity (target)

Isotype Control
Antibody type

Isotype Control
Host species

Mouse
Clonality

Monoclonal
Clone

IGG1/1331
Isotype

IgG1, kappa
Shipping condition

Room temperature
Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light, Note: store BSA-free antibodies at -10 to -35 °C
Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended
Regulatory status

For research use only (RUO)
Antibody application notes

For coating for ELISA, order Ab without BSA, Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody, Optimal dilution and staining procedure for a specific application should be determined by user, Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
Antibody/conjugate formulation

Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA, Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide
Product origin

Product may contain either bovine serum albumin (BSA) from bovine serum (Bos taurus), or recombinant BSA produced in Chinese hamster ovary cells. Inquire for the specific lot.

Technology

Primary & Secondary Antibody Conjugates

Tech Tips

Protocol: Fluorescent Western Blotting
Protocol: Immunofluorescence Staining of Cells for Microscopy
Tech Tip: Combined Direct and Indirect IF Using Primary Antibodies from the Same Host

Primary antibody formats:

  • CF® Dye, APC, R-PE, PerCP, biotin, and alkaline phosphatase conjugates: 0.1 mg/mL in PBS, 0.1% BSA, 0.05% sodium azide
  • Horseradish peroxidase: 0.1 mg/mL in PBS, 0.05% BSA, no sodium azide
  • Purified, with BSA: 0.2 mg/mL in PBS, 0.05% BSA, 0.05% sodium azide
  • Purified, BSA-free: 1 mg/mL in PBS, no BSA, no sodium azide
Protocols
SDS
Supporting Documents
Download Datasheet

Product Description

This is a non-reactive mouse monoclonal IgG1 kappa antibody labeled with one of our bright and photostable CF® Dyes and other labels. This antibody may serve as a control for a mouse monoclonal IgG1 antibody under identical experimental conditions. Biotium also carries mouse monoclonal isotype control antibodies for IgG2a Kappa (MG2a-53) and IgG2b Kappa (IGG1/1333).

  • Isotype control for antibody-based experiments
  • Available with bright and photostable CF® Dyes
  • APC, R-PE, RPE-Astral™616, APC-Astral™813, and biotin conjugates also available
  • Suitable for western, flow cytometry, ELISA, immunofluorescence,  and immunohistology

CF® Dyes offer exceptional brightness and photostability; learn more about CF® Dyes.

Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331)

Conjugation Ex/Em Size Catalog No. Dye Features
CF®405S 411/431 nm 250 uL (25 ug) BNC041331-250 CF®405S Features
1 mL (100 ug) BNC041331-1ML
CF®405L 413/547 nm 250 uL (25 ug) BNC061331-250 CF®405L Features
1 mL (100 ug) BNC061331-1ML
CF®488A 562/584 nm 250 uL (25 ug) BNC881331-250 CF®488A Features
1 mL (100 ug) BNC881331-1ML
CF®503R 503/532 nm 250 uL (25 ug) BNC501331-250 CF®503R Features
1 mL (100 ug) BNC501331-1ML
CF®514 516/549 nm 250 uL (25 ug) BNC511331-250 CF®514 Features
1 mL (100 ug) BNC511331-1ML
CF®568 562/584 nm 250 uL (25 ug) BNC681331-250 CF®568 Features
1 mL (100 ug) BNC681331-1ML
CF®575 575/601 nm 250 uL (25 ug) BNC571331-250
1 mL (100 ug) BNC571331-1ML
CF®583R 585/609 nm 250 uL (25 ug) BNC581331-250 CF®583R Features
1 mL (100 ug) BNC581331-1ML
CF®594 593/615 nm 250 uL (25 ug) BNC941331-250 CF®594 Features
1 mL (100 ug) BNC941331-1ML
RPE-Astral™616 496, 546, 566/617 nm 250 uL (25 ug) BNC161331-250
1 mL (100 ug) BNC161331-1ML
CF®640R 642/663 nm 250 uL (25 ug) BNC401331-250 CF®640R Features
1 mL (100 ug) BNC401331-1ML
CF®647 652/668 nm 250 uL (25 ug) BNC471331-250 CF®647 Features
1 mL (100 ug) BNC471331-1ML
CF®647Plus 652/668 nm 250 uL (25 ug) BNC641331-250
1 mL (100 ug) BNC641331-1ML
CF®660C 667/685 nm 250 uL (25 ug) BNC601331-250 CF®660C Features
1 mL (100 ug) BNC601331-1ML
CF®700 696/721 nm 250 uL (25 ug) BNC001331-250 CF®700 Features
1 mL (100 ug) BNC001331-1ML
APC-Astral™813 633, 638/813 nm 250 uL (25 ug) BNC131331-250
1 mL (100 ug) BNC131331-1ML
R-PE 496, 546, 565/576 nm 250 uL (25 ug) BNCR1331-250
1 mL (100 ug) BNCR1331-1ML
APC 651/660 nm 250 uL (25 ug) BNCA1331-250
1 mL (100 ug) BNCA1331-1ML
Biotin N/A 250 uL (25 ug) BNCB1331-250
1 mL (100 ug) BNCB1331-1ML
Purified N/A 250 uL (50 ug) BNUB1331-250
Purified, BSA-free N/A 50 uL (50 ug) BNUM1331-50
 Catalog number key for antibody number 1331, Anti-Isotype Control (IGG1/1331) 
Antibody # prefix Conjugation Ex/Em (nm) Laser line Detection channel Dye Features
BNC04 CF®405S 404/431 405 DAPI (microscopy), AF405 CF®405S Features
BNC88 CF®488A 490/515 488 GFP, FITC CF®488A Features
BNC68 CF®568 562/583 532, 561 RFP, TRITC CF®568 Features
BNC94 CF®594 593/614 561 Texas Red® CF®594 Features
BNC40 CF®640R 642/662 633-640 Cy®5 CF®640R Features
BNC47 CF®647 650/665 633-640 Cy®5 CF®647 Features
BNC74 CF®740 742/767 633-685 775/50 CF®740 Features
BNCB Biotin N/A N/A N/A
BNUB Purified N/A N/A N/A
BNUM Purified,
BSA-free		 N/A N/A N/A
Alexa Fluor, Pacific Blue, Pacific Orange, and Texas Red are trademarks or registered trademarks of Thermo Fisher Scientific; Cy is a registered trademark of Cytiva; IRDye, LI-COR, and Odyssey are registered trademarks of LI-COR Bioscience.