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Reagents and instruments for immunology, cell biology and molecular biology.

IL-5, recombinant Human

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Cat# NB-50-0179-1

Size : 1mg

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Phone : +1 850 650 7790

Brand : Neo Biotech

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Phone : +1 850 650 7790

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Bioassay analysis of Human IL-5 Recombinant Protein, PeproTech® (Product # 200-05-1MG).

Product Details

200-05-100UG

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

ELISA (ELISA)

Assay-dependent

Functional Assay (Functional)

Assay-dependent

In vitro Assay (IV)

View 30 publications 30 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species

Human

Published species

Guinea pig, Human, Mouse, Non-human primate

Expression System

E. coli

Amino acid sequence

MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES

Molecular weight

26.5 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

The ED50 as determined by the dose-dependent proliferation of TF-1 cells was ≤ 0.15 ng/ml, corresponding to a specific activity of ≥ 6.6 x 10^6 units/mg.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

Recombinant Human IL-5 is a 26.5 kDa disulfide-linked homodimeric protein containing two 116 amino acid chains.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5. IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

More than neutrophils: Lin(+)Ly6G(+)IL-5Rα(+) multipotent myeloid cells (MMCs) are dominant in normal murine bone marrow and retain capacity to differentiate into eosinophils and monocytes.

Journal of leukocyte biology

Jeong BM,Walker MT,Rodriguez R,Coden ME,Nagasaka R,Doan TC,Politanska Y,Abdala-Valencia H,Berdnikovs S

200-05 was used in In vitro experiments to discover an unexpected hematopoietic flexibility of a dominant population of multipotent myeloid cells, typically categorized as neutrophils, but with the previously unknown plasticity to contribute to mature pools of eosinophils and monocytes.

Sat Jan 01 00:00:00 UTC 2022

Identification of Keratinocyte Mitogens: Implications for Hyperproliferation in Psoriasis and Atopic Dermatitis.

JID innovations : skin science from molecules to population health

Niehues H,Rikken G,van Vlijmen-Willems IMJJ,Rodijk-Olthuis D,van Erp PEJ,Zeeuwen PLJM,Schalkwijk J,van den Bogaard EH

200-05 was used in In vitro experiments to investigate the mitogens involved in keratinocyte proliferation and stratification.

Sat Jan 01 00:00:00 UTC 2022

Cyclin D2 overexpression drives B1a-derived MCL-like lymphoma in mice.

The Journal of experimental medicine

Pieters T,T'Sas S,Vanhee S,Almeida A,Driege Y,Roels J,Van Loocke W,Daneels W,Baens M,Marchand A,Van Trimpont M,Matthijssens F,Morscio J,Lemeire K,Lintermans B,Reunes L,Chaltin P,Offner F,Van Dorpe J,Hochepied T,Berx G,Beyaert R,Staal J,Van Vlierberghe P,Goossens S

Mon Oct 04 00:00:00 UTC 2021

Severe T cell hyporeactivity in ventilated COVID-19 patients correlates with prolonged virus persistence and poor outcomes.

Nature communications

Renner K,Schwittay T,Chaabane S,Gottschling J,Müller C,Tiefenböck C,Salewski JN,Winter F,Buchtler S,Balam S,Malfertheiner MV,Lubnow M,Lunz D,Graf B,Hitzenbichler F,Hanses F,Poeck H,Kreutz M,Orsó E,Burkhardt R,Niedermair T,Brochhausen C,Gessner A,Salzberger B,Mack M

Fri May 21 00:00:00 UTC 2021

Microfilariae Trigger Eosinophil Extracellular DNA Traps in a Dectin-1-Dependent Manner.

Cell reports

Ehrens A,Lenz B,Neumann AL,Giarrizzo S,Reichwald JJ,Frohberger SJ,Stamminger W,Buerfent BC,Fercoq F,Martin C,Kulke D,Hoerauf A,Hübner MP

Tue Jan 12 00:00:00 UTC 2021

Identification of leukemic and pre-leukemic stem cells by clonal tracking from single-cell transcriptomics.

Nature communications

Velten L,Story BA,Hernández-Malmierca P,Raffel S,Leonce DR,Milbank J,Paulsen M,Demir A,Szu-Tu C,Frömel R,Lutz C,Nowak D,Jann JC,Pabst C,Boch T,Hofmann WK,Müller-Tidow C,Trumpp A,Haas S,Steinmetz LM

Mon Mar 01 00:00:00 UTC 2021

A comprehensive single cell transcriptional landscape of human hematopoietic progenitors.

Nature communications

Pellin D,Loperfido M,Baricordi C,Wolock SL,Montepeloso A,Weinberg OK,Biffi A,Klein AM,Biasco L

Mon Jun 03 00:00:00 UTC 2019

Lin- CD34hi CD117int/hi FcεRI+ cells in human blood constitute a rare population of mast cell progenitors.

Blood

Dahlin JS,Malinovschi A,Öhrvik H,Sandelin M,Janson C,Alving K,Hallgren J

Thu Jan 28 00:00:00 UTC 2016

Cytokine-Induced Glucocorticoid Resistance from Eosinophil Activation: Protein Phosphatase 5 Modulation of Glucocorticoid Receptor Phosphorylation and Signaling.

Journal of immunology (Baltimore, Md. : 1950)

Pazdrak K,Straub C,Maroto R,Stafford S,White WI,Calhoun WJ,Kurosky A

Tue Nov 15 00:00:00 UTC 2016

Failed CTL/NK cell killing and cytokine hypersecretion are directly linked through prolonged synapse time.

The Journal of experimental medicine

Jenkins MR,Rudd-Schmidt JA,Lopez JA,Ramsbottom KM,Mannering SI,Andrews DM,Voskoboinik I,Trapani JA

Mon Mar 09 00:00:00 UTC 2015

Cytokine-stimulated Phosphoflow of PBMC Using CyTOF Mass Cytometry.

Bio-protocol

Fernandez R,Maecker H

200-05 was used in Cell Culture to use phosphoprotein-specific antibodies with flow cytometry to study the level of phosphorylation of intracellular proteins in response to stimulation by cytokines, and use this process to gain insight to the status of the immune system.

Fri Jun 05 00:00:00 UTC 2015

TGF-β-inducible microRNA-183 silences tumor-associated natural killer cells.

Proceedings of the National Academy of Sciences of the United States of America

Donatelli SS,Zhou JM,Gilvary DL,Eksioglu EA,Chen X,Cress WD,Haura EB,Schabath MB,Coppola D,Wei S,Djeu JY

Tue Mar 18 00:00:00 UTC 2014

Eosinophils regulate peripheral B cell numbers in both mice and humans.

Journal of immunology (Baltimore, Md. : 1950)

Wong TW,Doyle AD,Lee JJ,Jelinek DF

Tue Apr 15 00:00:00 UTC 2014

Peptide library-based evaluation of T-cell receptor breadth detects defects in global and regulatory activation in human immunologic diseases.

Proceedings of the National Academy of Sciences of the United States of America

Barber JS,Yokomizo LK,Sheikh V,Freeman AF,Garabedian E,van Dijk E,Sokolic R,Candotti F,Weng NP,Sereti I,Milner JD

Tue May 14 00:00:00 UTC 2013

Adam8 limits the development of allergic airway inflammation in mice.

Journal of immunology (Baltimore, Md. : 1950)

Knolle MD,Nakajima T,Hergrueter A,Gupta K,Polverino F,Craig VJ,Fyfe SE,Zahid M,Permaul P,Cernadas M,Montano G,Tesfaigzi Y,Sholl L,Kobzik L,Israel E,Owen CA

Sat Jun 15 00:00:00 UTC 2013

Rapid and unidirectional perforin pore delivery at the cytotoxic immune synapse.

Journal of immunology (Baltimore, Md. : 1950)

Lopez JA,Jenkins MR,Rudd-Schmidt JA,Brennan AJ,Danne JC,Mannering SI,Trapani JA,Voskoboinik I

Sun Sep 01 00:00:00 UTC 2013

Monocyte-derived IL-5 reduces TNF production by Mycobacterium tuberculosis-specific CD4 T cells during SIV/M. tuberculosis coinfection.

Journal of immunology (Baltimore, Md. : 1950)

Diedrich CR,Mattila JT,Flynn JL

Sat Jun 15 00:00:00 UTC 2013

The IL-7Rα pathway is quantitatively and functionally altered in CD8 T cells in multiple sclerosis.

Journal of immunology (Baltimore, Md. : 1950)

Kreft KL,Verbraak E,Wierenga-Wolf AF,van Meurs M,Oostra BA,Laman JD,Hintzen RQ

Wed Feb 15 00:00:00 UTC 2012

Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells.

Nature protocols

Choi KD,Vodyanik M,Slukvin II

200-05 was used in Cell Culture to study how differentiation of myeloid progenitors into mature myeloid cells requires an additional 5-19 d of culture with cytokines, depending on the cell type.

Tue Mar 01 00:00:00 UTC 2011

Hematopoietic differentiation and production of mature myeloid cells from human pluripotent stem cells.

Nature protocols

Choi KD,Vodyanik M,Slukvin II

200-05 was used in Cell Culture to study how differentiation of myeloid progenitors into mature myeloid cells requires an additional 5-19 d of culture with cytokines, depending on the cell type.