IgG, Kappa (HRP)

Applications: |Suitable for use in Immunoblot (Western and Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications not tested.||Recommended Dilutions:|Western Blot: 1:2000-1:5000|ELISA: 1:50,000|Immunohistochemistry: 1:500-1-2500|Optimal dilutions to be determined by the researcher.||Storage and Stability:|Lyophilized powder is stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20°C. Reconstituted product is stable for 12 months at -20°C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.