Hydrogen Peroxide (H2O2) Colorimetric Assay Kit

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Cat# E-BC-K102-S-100

Size : 100Assays

Contact local distributor :

Phone : +1 850 650 7790

Brand : Elabscience

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Contact local distributor :

Phone : +1 850 650 7790

Detection Principle	Hydrogen peroxide can react with ammonium molybdate to form a yellow complex which has a maximum absorption peak at 405 nm. H₂O₂ content can be calculated by measuring the absorbance value at 405 nm.
Sample Type	Serum,Plasma,Cell culture supernatant,Tissue,Cell
Detection Method	Colorimetric method
Detection Instrument	Spectrophotometer (405 nm)
Research Area	Oxidative Stress , Plant Physiology Series , Ferroptosis
Other Reagents Required	Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4)
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1.5 mmol/L
Detection Range	1.5-150 mmol/L
Precision	inter-assay CV: 2.7% \| intra-assay CV: 1.3%
Sample Volume	0.1 mL
Assay Time	1 h

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mouse liver tissue homogenization	5-10
10% Green pepper tissue homogenization	1
Human serum	1
Rat serum	1
Mouse serum	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.