Human IgG1 monoclonal antibody, clone RM117 (Biotin)

Catalog # MAB14956
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Size:50 ug
Price: USD $ 428.00
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Enzyme-linked Immunoabsorbent Assay
Application

Enzyme-linked Immunoabsorbent Assay

ELISA analysis of Human IgG1 monoclonal antibody, clone RM117 (Cat # MAB12818) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgG1; no cross reactivity human IgG2, IgG3, IgG4, IgE, IgA, or IgD, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

  • Specifications

    Product Description

    Rabbit recombinant monoclonal antibody raised against of human IgG1.Recombinant Antibody,Recombinant Antibodies,Recombinant Monoclonal Antibody,RecomAb,Recombinant Ab,Recombinant Monoclonal Antibodies,Recombinant Abs

    Antibody Species

    Rabbit

    Immunogen

    Original antibody is raised against a synthetic peptide corresponding to the hinge region of human IgG1.

    Sequence

    N/A

    Reactivity

    Human

    Form

    Liquid

    Conjugation

    Biotin

    Purification

    Protein A purification

    Isotype

    IgG

    Recommend Usage

    ELISA (0.05-0.2 ug/well for capture, 0.05-0.2 ug/mL for detection)
    Flow Cytometry
    Immunocytochemistry (0.5-2 ug/mL)
    Immunohistochemistry (0.5-2 ug/mL)
    The optimal working dilution should be determined by the end user.

    Storage Buffer

    In PBS (50% glycerol, 1% BSA, 0.09% sodium azide)

    Storage Instruction

    Store at -20°C.
    Aliquot to avoid repeated freezing and thawing.

    Note

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

  • Applications

    Immunohistochemistry

    Immunocytochemistry

    Enzyme-linked Immunoabsorbent Assay

    ELISA analysis of Human IgG1 monoclonal antibody, clone RM117 (Cat # MAB12818) at the following concentrations: 0.2 ug/mL, 0.05 ug/mL, 0.01 ug/mL. The plate was coated with 50 ng/well of different immunoglobulins and the result shows this antibody reacts to human IgG1; no cross reactivity human IgG2, IgG3, IgG4, IgE, IgA, or IgD, mouse IgG, rat IgG, or goat IgG. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

    Flow Cytometry

  • Gene Info — IGHG1

    Entrez GeneID

    3500

    Protein Accession#

    P01857

    Gene Name

    IGHG1

    Gene Alias

    -

    Gene Description

    immunoglobulin heavy constant gamma 1 (G1m marker)

    Omim ID

    147100

    Gene Ontology

    Hyperlink

    Other Designations

    -

  • Interactomes
  • Diseases