Reagents and instruments for immunology, cell biology and molecular biology.
 
> Human Cortisol (saliva) ELISA Kit, 96 tests, Quantitative

Human Cortisol (saliva) ELISA Kit, 96 tests, Quantitative

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Cat# 3500

Size : 1kit

Brand : Alpha Diagnostic International

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Phone : +1 850 650 7790

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KitContents1. Anti-Cortisol coated strip plate (96 wells), Cat. 3501 1 plate 2. Cortisol Saliva Std. A (0 ng/ml), 10 ml , Cat # 3502A 1 vial 3. Cortisol Saliva Std. B (1 ng/ml), 0.6 ml , Cat # 3502B 1 vial 4. Cortisol Saliva Std. C (3 ng/ml), 0.6 ml , Cat # 3502C 1 vial 5. Cortisol Saliva Std. D (10 ng/ml), 0.6 ml , Cat # 3502D 1 vial 6. Cortisol Saliva Std. E (30 ng/ml), 0.6 ml , Cat # 3502E 1 vial 7. Cortisol Saliva Std. F (100 ng/ml), 0.6 ml , Cat # 3502F 1 vial 8. Cortisol Saliva Control Serum, 0.6 ml; Cat # 3503 (refer to concn on the vial) 1 vial 9. Assay buffer, 15 ml; Cat # 3504 1 bottle 10. Cortisol-HRP Conjugate (50X), 300 ul Cat # 3505,Dilute 1:50 in assay buffer before use, 1 bottle 11. TMB substrate, 16 ml, Cat # 3506 1 bottle 12. Stop solution (1N H2SO4), 6 ml; Cat. # T-30 1 bottle 13. Wash buffer (10X), 50 ml, Dilute 1:10, WB-10 1 bottle 14. Instruction Manual, M-3500 1
MSDSTMB (substrate), H2SO4 (1% in a buffered stop solution), and Prolcin-300 (0.1% v/v in standards, sample diluent and HRP-conjugates
Product TypeELISA Kit
SamplesSerum. Other fluids not optimized.
SpCrossreactivityThe following compounds were tested for cross-reactivity with the Direct Cortisol Saliva ELISA kit with cortisol cross-reacting at 100%; Cortisol, 100%, Prednisolone (13%), Corticosterone, Deoxycorticosterone, Progesterone, Cortisone, Deoxycortisol, Pednisone (5-7%), Dexamethasone (1-2%). No cross reaction was detected with DHEAS and Tetrahydrocortisone. Human cortisol kit has been designed and tested for human samples. Another kit for human serum or plasma is available. It has not been tested in animals (rat, mouse, etc). Since the steroid hormone is the same in many species, this kit should work in most species as long as the steroid concentration is within the range of this kit.
SpeciesReactivityAll species

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