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> Goat Anti-Human Kappa-TRITC

Goat Anti-Human Kappa-TRITC

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Cat# 2060-03

Size : 1.0mg

Brand : Southern Biotech

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Phone : +1 850 650 7790

Product Specifications

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Isotype Goat IgG
Isotype Control Goat IgG-TRITC
Specificity Reacts with human κ light chains
Source Pooled antisera from goats hyperimmunized with human κ light chains
Cross Adsorption Human λ light chains; may react with κ light chains from other species
Purification Method Affinity chromatography on human κ light chains covalently linked to agarose
Conjugate TRITC (Rhodamine)
Buffer Formulation Phosphate buffered saline containing < 0.1% sodium azide
Clonality Polyclonal
Concentration 1.0 mg/mL
Volume 1.0 mL
Recommended Storage 2-8°C; Avoid exposure to light
Applications Quality tested applications for relevant formats include -
ELISA 1-9
FLISA
Flow Cytometry 19-22

Other referenced applications for relevant formats include -
ELISpot 10
Immunohistochemistry-Paraffin Sections 11
Immunocytochemistry 12
Western Blot 4,13-17
Immunoprecipitation 18
Surface Plasmon Resonance 23
Purification 24
Stimulation 25
RRID Number AB_2795718
Gene ID 3514 (Human)
Gene ID Symbol IGKC (Human)
Gene ID Aliases HCAK1D; Km; IGKC
UniProt ID P01834 (Human
UniProt Name IGKC_HUMAN (Human)

Documentation

Documentation
Technical Bulletin Safety Datasheet
Certificate of Analysis Lookup

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Other Conjugates and Formulations

UNLB FITC AP HRP BGAL TXRD BIOT PE AF488 AF647 AF555

Citations

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  2. 2. Akamatsu Y, Pakabunto K, Xu Z, Zhang Y, Tsurushita N. Whole IgG surface display on mammalian cells: Application to isolation of neutralizing chicken monoclonal anti-IL-12 antibodies. J Immunol Methods. 2007;327:40-52. (ELISA)
  3. 3. Guan Y, Sajadi MM, Kamin-Lewis R, Fouts TR, Dimitrov A, Zhang Z, et al. Discordant memory B cell and circulating anti-Env antibody responses in HIV-1 infection. Proc Natl Acad Sci USA. 2009;106:3952-7. (ELISA)
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  5. 5. Boross P, Lohse S, Nederend M, Jansen JH, van Tetering G, Dechant M, et al. IgA EGFR antibodies mediate tumour killing in vivo. EMBO Mol Med. 2013;5:1213-26. (ELISA)
  6. 6. Kehoe JW, Whitaker B, Bethea D, Lacy ER, Boakye K, Santulli-Marotto S, et al. Isolation and optimization for affinity and biophysical characteristics of anti-CCL17 antibodies from the VH1-69 germline gene. Protein Eng Des Sel. 2014;27:199-206. (ELISA)
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  8. 8. Nagano R, Masuda K. Establishment of a signal peptide with cross-species compatibility for functional antibody expression in both Escherichia coli and Chinese hamster ovary cells. Biochem Biophys Res Commun. 2014;447:655-9. (ELISA)
  9. 9. London AS, Mackay K, Lihon M, He Y. Gel filtration chromatography as a method for removing bacterial endotoxin from antibody preparations. Biotechnol Prog. 2014;30:1497-1501. (ELISA)
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  12. 12. Campana D, Farrant J, Inamdar N, Webster AD, Janossy G. Phenotypic features and proliferative activity of B cell progenitors in X-linked agammaglobulinemia. J Immunol. 1990;145:1675-80. (ICC)
  13. 13. Singh G, Parker S, Hobart P. The development of a bicistronic plasmid DNA vaccine for B-cell lymphoma. Vaccine. 2002;20:1400-11. (WB)
  14. 14. Corisdeo S, Wang B. Functional expression and display of an antibody Fab fragment in Escherichia coli: study of vector designs and culture conditions. Protein Expr Purif. 2004;34:270-9. (WB)
  15. 15. Phoolcharoen W, Bhoo SH, Lai H, Ma J, Arntzen CJ, Chen Q, et al. Expression of an immunogenic Ebola immune complex in Nicotiana benthamiana. Plant Biotechnol J. 2011;9:807-16. (WB)
  16. 16. He J, Peng L, Lai H, Hurtado J, Stahnke J, Chen Q. A plant-produced antigen elicits potent immune responses against West Nile virus in mice. Biomed Res Int. 2014;2014:952865. (WB)
  17. 17. Helman D, Toister-Achituv M, Bar-Shimon M, Cohen B, Otmi I, Berger N, et al. Novel membrane-bound reporter molecule for sorting high producer cells by flow cytometry. Cytometry A. 2014;85:162-8. (WB)
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  22. 22. Xiang Y, Park S, Garrard WT. A major deletion in the V-J intervening region results in hyperelevated transcription of proximal V genes and a severely restricted repertoire. J Immunol. 2014;193:3746-54. (FC)
  23. 23. Wagner K, Kwakkenbos MJ, Claassen YB, Maijoor K, Böhne M, van der Sluijs KF, et al. Bispecific antibody generated with sortase and click chemistry has broad antiinfluenza virus activity. Proc Natl Acad Sci USA. 2014;111:16820-5. (Surface Plasmon Resonance)
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