Free Cholesterol (FC) Colorimetric Assay Kit

Print

Cat# E-BC-K004-M-48T

Size : 48T

Contact local distributor :

Phone : +1 850 650 7790

Brand : Elabscience

Request more information

Contact local distributor :

Phone : +1 850 650 7790

Detection Principle	Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content.
Synonyms	FC
Sample Type	Serum,Plasma,Tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (495-525 nm, optimum wavelength: 510 nm)
Research Area	Glycolysis And Lipid Metabolism
Other Reagents Required	Ethanol absolute
Storage	This product can be stored at 2-8°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	0.07 mmol/L
Detection Range	0.07-24 mmol/L
Precision	inter-assay CV: 5.2% \| intra-assay CV: 1.9%
Sample Volume	5 μL
Assay Time	20 min

Cat.No.	Product Name	Sizes
E-BC-K109-M	Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)	500Assays , 96T , 48T
E-BC-K205-M	Low-Density Lipoprotein Cholesterol (LDL-C) Colorimetric Assay Kit (Double Reagents)	96T
E-BC-K221-M	High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents)	96T
E-BC-K261-M	Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)	500Assays , 96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
Human plasma	1
Human serum	1
Rat serum	1
Mouse serum	1
Rabbit serum	1
10% Rat kidney tissue homogenate	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.