FlyCut^® Eagl is expressed and purified from E.coli that carries the recombinant Eagl gene. The molecular weight is 35.1 kDa, with the recognition site at C^GGCCG. The reaction is conducted for 5-15 minutes at 37°C, and heat-inactivated at 65°C for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation. 

Enzyme Properties 

Fast digestion in 5-15 minutes with high fidelity 

Application 

Genomic DNA, plasmid DNA, PCR product