Ferrous Iron Colorimetric Assay Kit

Cat# E-BC-K304-S-50

Size : 50Assays

Brand : Elabscience

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Phone : +1 850 650 7790

Detection Principle Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.
Sample Type Serum,Plasma,Animal tissue
Detection Method Colorimetric method
Detection Instrument Spectrophotometer (520 nm)
Research Area Inorganic Ions ,  Plant Physiology Series ,  Ferroptosis
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 0.08 mg/L
Detection Range 0.08-20 mg/L
Precision inter-assay CV: 3.2% | intra-assay CV: 0.03
Sample Volume 0.5 mL
Assay Time 45 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human plasma 1
Human serum 1
10% Rat liver tissue homogenate 1
10% Epipremnum aureum homogenate 1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

Other Clones

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Other Formats

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Biotin

Chromogenic Biotin

Cyanine 3

Cyanine 5

Cyanine 5.5

Cyanine 7

Elab Fluor® Violet 450

Elab Fluor®430

Elab Fluor®488

Elab Fluor®568

Elab Fluor®594

Elab Fluor®647

Elab Fluor®680

Elab Fluor®700

Elab Fluor®750

Elab Fluor®Red 780

Elab Fluor®Violet 450

FITC

HRP

NHS-Biotin

NHS-LC-LC-Biotin

Sulfo-NHS-Biotin

Sulfo-NHS-LC-LC-Biotin

Unconjugated

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detection instrument

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