Exosomes are a class of vesicle-like structures with a diameter of about 30-150 nm, which are released into the extracellular environment by cells through exocytosis. They are found in the body fluids of almost all living organisms, including blood, urine, saliva, milk, and cell supernatants cultured in vitro. Exosomes are important tools for cell-to-cell communication, which can carry and deliver a variety of biomolecules such as proteins, lipids, RNA, and DNA, thus playing an important role in regulating immune responses, promoting tissue repair, and transmitting genetic information.

Exosome Isolation Kit for Cell Culture Media is a kit for efficient, simple, and rapid extraction of exosomes from cell culture medium using polymer precipitation. This kit does not require ultracentrifugation or ultrafiltration, and a large number of intact and high-purity exosomes can be obtained directly from cell culture supernatants with a simple lower centrifugation. This kit has low equipment requirements, takes less time, and is free of RNases/DNases/proteases. The exosomes extracted using this kit can be used for protein analysis, nucleic acid analysis, Western Blot, PCR, RNA extraction, high-throughput sequencing, exosome particle size and concentration analysis (Nanoparticle tracing analysis, NTA), electron microscopy analysis, and cell co-culture.