CRMP5 (DPYSL5) (NM_020134) Human Tagged ORF Clone

Cat# RC202631

Size : 10ug

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CRMP5 (DPYSL5) (NM_020134) Human Tagged ORF Clone

SKU
RC202631
DPYSL5 (Myc-DDK-tagged)-Human dihydropyrimidinase-like 5 (DPYSL5)
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    Protein expression verified by Western blot, fully sequenced and in stock

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Specifications
Product Data
Target Symbol CRMP5
Synonyms CRAM; CRMP-5; CRMP5; CV2; Ulip6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202631 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTGCCAACTCAGCCAGCGTGAGGATCCTCATCAAGGGAGGCAAGGTGGTGAACGATGACTGCACCC
ACGAGGCTGACGTCTACATCGAGAATGGCATCATCCAGCAGGTGGGCCGCGAGCTCATGATCCCTGGCGG
GGCCAAGGTGATTGATGCCACAGGAAAACTGGTGATCCCTGGTGGCATCGACACCAGCACCCACTTCCAC
CAGACCTTCATGAATGCCACGTGCGTGGACGACTTCTACCATGGGACCAAGGCAGCACTCGTCGGAGGCA
CCACCATGATCATCGGCCACGTCCTGCCCGACAAGGAGACCTCCCTTGTGGACGCTTATGAGAAGTGCCG
AGGTCTGGCCGACCCCAAGGTCTGCTGTGATTACGCCCTCCACGTGGGGATCACCTGGTGGGCACCCAAG
GTGAAAGCAGAAATGGAGACACTGGTGAGGGAGAAGGGTGTCAACTCGTTCCAGATGTTCATGACCTACA
AGGACCTGTACATGCTTCGAGACAGTGAGCTGTACCAAGTGTTGCACGCTTGCAAGGACATTGGGGCAAT
CGCCCGCGTCCATGCTGAAAATGGGGAGCTTGTGGCCGAGGGTGCTAAGGAGGCACTGGATTTGGGGATC
ACAGGCCCAGAAGGAATCGAGATCAGCCGTCCAGAGGAGCTGGAAGCTGAAGCCACTCATCGTGTTATCA
CCATTGCAAACAGGACTCACTGTCCAATCTACCTGGTCAACGTGTCCAGTATCTCGGCTGGTGACGTTAT
CGCAGCTGCTAAGATGCAAGGGAAGGTTGTGCTGGCGGAGACCACCACTGCACATGCCACGCTGACAGGC
TTACACTACTACCACCAGGACTGGTCCCACGCGGCTGCCTATGTCACGGTGCCTCCCCTGAGACTGGACA
CCAACACCTCAACCTACCTCATGAGCCTGCTGGCCAATGACACTCTGAACATCGTGGCATCAGATCACCG
GCCTTTCACCACAAAGCAGAAAGCTATGGGCAAGGAAGACTTCACCAAGATCCCACATGGAGTGAGTGGC
GTGCAGGACCGCATGAGCGTCATCTGGGAGAGAGGAGTGGTTGGAGGAAAGATGGATGAGAACCGTTTTG
TGGCCGTTACCAGTTCCAACGCAGCTAAGCTTCTGAACCTGTATCCCCGCAAGGGCCGCATTATTCCCGG
AGCCGATGCTGATGTGGTGGTGTGGGACCCAGAAGCCACAAAGACCATCTCAGCCAGCACGCAGGTCCAG
GGAGGAGACTTCAACCTGTATGAGAACATGCGCTGCCACGGCGTGCCACTGGTCACCATCAGCCGGGGGC
GCGTCGTGTATGAGAACGGCGTCTTCATGTGCGCCGAGGGCACCGGCAAGTTCTGTCCCCTGAGGTCCTT
CCCAGACACTGTCTACAAGAAGCTGGTCCAGAGAGAGAAGACTTTAAAGGTTAGAGGAGTGGACCGCACT
CCCTACCTGGGGGATGTCGCTGTTGTCGTGCACCCTGGGAAAAAAGAGATGGGAACCCCACTCGCAGACA
CTCCTACCCGGCCCGTCACCCGGCATGGGGGCATGAGGGACCTTCACGAATCCAGCTTCAGCCTCTCTGG
CTCTCAGATCGATGACCATGTTCCAAAGCGAGCTTCAGCTCGGATCCTCGCTCCTCCCGGAGGCAGGTCG
AGTGGCATTTGG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC202631 protein sequence
Red=Cloning site Green=Tags(s)

MLANSASVRILIKGGKVVNDDCTHEADVYIENGIIQQVGRELMIPGGAKVIDATGKLVIPGGIDTSTHFH
QTFMNATCVDDFYHGTKAALVGGTTMIIGHVLPDKETSLVDAYEKCRGLADPKVCCDYALHVGITWWAPK
VKAEMETLVREKGVNSFQMFMTYKDLYMLRDSELYQVLHACKDIGAIARVHAENGELVAEGAKEALDLGI
TGPEGIEISRPEELEAEATHRVITIANRTHCPIYLVNVSSISAGDVIAAAKMQGKVVLAETTTAHATLTG
LHYYHQDWSHAAAYVTVPPLRLDTNTSTYLMSLLANDTLNIVASDHRPFTTKQKAMGKEDFTKIPHGVSG
VQDRMSVIWERGVVGGKMDENRFVAVTSSNAAKLLNLYPRKGRIIPGADADVVVWDPEATKTISASTQVQ
GGDFNLYENMRCHGVPLVTISRGRVVYENGVFMCAEGTGKFCPLRSFPDTVYKKLVQREKTLKVRGVDRT
PYLGDVAVVVHPGKKEMGTPLADTPTRPVTRHGGMRDLHESSFSLSGSQIDDHVPKRASARILAPPGGRS
SGIW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_020134
ORF Size 1692 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at info@biovalley.fr

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020134.4
RefSeq Size 5225 bp
RefSeq ORF 1695 bp
Locus ID 56896
UniProt ID Q9BPU6
Cytogenetics 2p23.3
Domains Amidohydro_1
Protein Pathways Axon guidance
MW 61.4 kDa
Summary This gene encodes a member of the CRMP (collapsing response mediator protein) family thought to be involved in neural development. Antibodies to the encoded protein were found in some patients with neurologic symptoms who had paraneoplastic syndrome. A pseudogene of this gene is found on chromosome 11. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Dec 2011]
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You're reviewing:CRMP5 (DPYSL5) (NM_020134) Human Tagged ORF Clone
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SKU Description Size
RC202631L3 Lenti ORF clone of Human dihydropyrimidinase-like 5 (DPYSL5), Myc-DDK-tagged 10 ug
RC202631L4 Lenti ORF clone of Human dihydropyrimidinase-like 5 (DPYSL5), mGFP tagged 10 ug
RG202631 DPYSL5 (tGFP-tagged) - Human dihydropyrimidinase-like 5 (DPYSL5) 10 ug
SC113189 DPYSL5 (untagged)-Human dihydropyrimidinase-like 5 (DPYSL5) 10 ug
SC320721 DPYSL5 (untagged)-Human dihydropyrimidinase-like 5 (DPYSL5) 10 ug
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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