Cell Total Iron Colorimetric Assay Kit
Cat# E-BC-K880-M-48T
Size : 48T
Brand : Elabscience
Contact local distributor :
Phone : +1 850 650 7790
| Detection Principle | Iron is one of the metal elements in organism and has important physiological functions. Ferrous ion is a key element in heme and hemoglobin and plays an important role in many biochemical reactions. Under the action of reductant, iron ions in samples can be reduced into ferrous ions (Fe2+). The latter then bind to probe and form complexes, which has a maximum absorption peak at 593 nm. The concentration of iron can be calculated by measuring the OD value at 593 nm indirectly. |
| Synonyms | Fe |
| Sample Type | Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (590-600 nm, optimum wavelength: 593 nm） |
| Research Area | Inorganic Ions , Ferroptosis |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.4 μmol/L |
| Detection Range | 0.4-50 μmol/L |
| Precision | inter-assay CV: 1.5% | intra-assay CV: 1.3% |
| Sample Volume | 80 μL(cell homogenate) |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| HepG2 Cell | 1 |
| molt-4 Cell | 1 |
| Jurkat Cell | 1 |
| HEL Cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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Biotin
Chromogenic Biotin
Cyanine 3
Cyanine 5
Cyanine 5.5
Cyanine 7
Elab Fluor® Violet 450
Elab Fluor®430
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Unconjugated
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