Flow Cytometry. (Ref.1,5) Immunoprecipitation. (Ref.1) Functional Assay:In vivo and in vitro blockage of CD154 function. (Ref.1,3,5)
Reactivity
Mouse
Antibody Host
Hamster
Isotype
IgG
Clonality
Monoclonal
Immunogen
Activated mouse Th1 clone D1.6 (Ref.1)
Specificity
This antibody is specific to CD154 (CD40 ligand/gp39), Mr 39-kDa. The MR1 monoclonal antibody binds to murine CD154 with high affinity, blocks binding to CD40, and blocks CD154 function. (Ref.1,5) Administration of this antibody to mice blocks the ability to mount primary and secondary immune responses to TD antigens, yet does not alter the immune response to TI antigens. (Ref.4)
CD154, formerly known as CD40 ligand and gp39, is a type II integral membrane protein and a member of the tumor necrosis factor (TNF) family of ligands. (Ref.1-7) It is an important accessory molecule in T cell-B cell costimulatory interactions, and is expressed predominantly on activated CD4+ T lymphocytes. It is also present on the surface of activated Th0, Th1, and Th2 T cell clones. Its expression is transient and cyclosporinsensitive. (Ref.6)
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