CA 15-3 ELISA Kit
Cat# EA101114
Size : 1kit
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| Product Data | |
| Format | 96-well strip plate |
|---|---|
| Assay Type | Solid Phase Sandwich ELISA |
| Assay Length | 3 hour |
| Signal | Colorimetric |
| Sample Type | Cell culture supernatant, serum, plasma |
| Sample Volume | 10 ul |
| Specificity | Natural and recombinant human CA 15-3 |
| Reactivity | Human |
| Interference | No significant interference observed with available related molecules. |
| Components | See the product information for more details |
| Background | The CA 15-3 ELISA Kit is a solid phase enzyme-linked immunosorbent assay (ELISA) based on the sandwich principle. The microtiter wells are coated with a mouse monoclonal antibody directed towards a unique antigenic site of the CA15-3 molecule. An aliquot of sample containing endogenous CA15-3 is incubated in the coated well with enzyme conjugate, which is an anti-CA15-3 antibody conjugated with horseradish peroxidase. After incubation the unbound conjugate is washed off. The amount of bound peroxidase is proportional to the concentration of CA15-3 in the sample. Having added the substrate solution, the intensity of colour developed is proportional to the concentration of CA15-3 in the sample. |