Anti-RFP (GOAT) Antibody
Cat# 200-101-379
Size : 100ug
Brand : Rockland Immunochemicals
Contact local distributor :
Phone : +1 850 650 7790
Specifications for RFP Antibody
Product Details
Anti-RFP (GOAT) Antibody - 200-101-379
goat anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
Goat
Polyclonal
IgG
Target Details
DsRed - View All DsRed Products
RFP, rRFP
Recombinant Protein
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Anti-RFP (GOAT) is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.
Q9U6Y8 - UniProtKB
Application Details
WB
EM, FC, IF, IHC, Multiplex - View References
Polyclonal anti-RFP has been tested by and western blot and is designed to detect RFP and its variants. Optimal titers for applications should be determined by the researcher. Expect a band ~27kDa in Western Blot in the appropriate cell lysate or extract.
Formulation
1 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Background
Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, immunofluorescence, and fluorescence activated cell sorting (FACS).
References (55)
Cathomas F et al. (2024). Circulating myeloid-derived MMP8 in stress susceptibility and depression. Nature.
Applications
IHC, ICC, Histology PubMed
Amato Jr E et al. (2024). Illuminating the terminal nerve: Uncovering the link between GnRH-1 neuron and olfactory development. J Comp Neurol.
Applications
IHC, ICC, Histology PubMed
Recinto SJ et al. (2024). Characterizing enteric neurons in dopamine transporter (DAT)-Cre reporter mice reveals dopaminergic subtypes with dual-transmitter content. Eur J Neurosci.
Applications
IF, Confocal Microscopy PubMed
Haist KC et al. (2024). A LTB4/CD11b self-amplifying loop drives pyogranuloma formation in chronic granulomatous disease. iScience.
Applications
IF, Confocal Microscopy PubMed
Curran BM et al. (2024). Multiple Guidance Mechanisms Control Axon Growth to Generate Precise T-shaped Bifurcation during Dorsal Funiculus Development in the Spinal Cord. bioRxiv [Preprint].
Applications
IF, Confocal Microscopy PubMed
Ciceri G et al. (2024). An epigenetic barrier sets the timing of human neuronal maturation. Nature.
Applications
IHC, ICC, Histology PubMed
Liu K et al. (2024). Tracing the origin of alveolar stem cells in lung repair and regeneration. Cell.
Applications
IF, Confocal Microscopy PubMed
Nam HS et al. (2023). Lrig1 expression identifies quiescent stem cells in the ventricular‑subventricular zone from postnatal development to adulthood and limits their persistent hyperproliferation. Neural Dev.
Applications
IF, Confocal Microscopy PubMed
Zhao H et al (2023). Use of a dual genetic system to decipher exocrine cell fate conversions in the adult pancreas. Cell Discov.
Applications
IF, Confocal Microscopy PubMed
Ota R et al. (2023). Cortical projection to the subventricular zone and its effect on adult neurogenesis in mice. Neurosci Lett.
Applications
IF, Confocal Microscopy PubMed
Masuda T et al. (2023). Pancreatic RECK inactivation promotes cancer formation, epithelial-mesenchymal transition, and metastasis. J Clin Invest.
Applications
IHC, ICC, Histology PubMed
Noack F et al. (2023). Joint epigenome profiling reveals cell-type-specific gene regulatory programmes in human cortical organoids. Nat Cell Biol.
Applications
IHC, ICC, Histology PubMed
Rashid M et al. (2023). Inhibition of high-fat diet-induced inflammatory responses in adipose tissue by SF1-expressing neurons of the ventromedial hypothalamus. Cell Rep,
Applications
IF, Confocal Microscopy PubMed
Deng L et al. (2023). Nav1.7 is essential for nociceptor action potentials in the mouse in a manner independent of endogenous opioids. Neuron.
Applications
IHC, ICC, Histology PubMed
Pu W et a. (2023). Bipotent transitional liver progenitor cells contribute to liver regeneration. Nat Genet
Applications
IF, Confocal Microscopy PubMed
Wang J et al. (2023). An ultra-compact promoter drives widespread neuronal expression in mouse and monkey brains. Cell Rep.
Applications
IF, Confocal Microscopy PubMed
Liu L et al. (2023). Modeling post-implantation stages of human development into early organogenesis with stem-cell-derived peri-gastruloids. Cell.
Applications
IF, Confocal Microscopy PubMed
Lauer SM et al. (2023). Recruitment of BAG2 to DNAJ-PKAc scaffolds promotes cell survival and resistance to drug-induced apoptosis in fibrolamellar carcinoma. bioRxiv [Preprint].
Applications
IF, Confocal Microscopy PubMed
Salery M et al. (2023). Transcriptional correlates of cocaine-associated learning in striatal ARC ensembles. bioRxiv [Preprint].
Applications
IHC, ICC, Histology PubMed
Sato Y et al. (2023). Aquaporin regulates cell rounding through vacuole formation during endothelial-to-hematopoietic transition. Development.
Applications
IF, Confocal Microscopy PubMed
Chen APF et al. (2023). Nigrostriatal dopamine modulates the striatal-amygdala pathway in auditory fear conditioning. Nat Commun.
Applications
IHC, ICC, Histology PubMed
Liu K et al. (2023). Intercellular genetic tracing of cardiac endothelium in the developing heart. Dev Cell.
Applications
IF, Confocal Microscopy PubMed
Hayes BH et al. (2023). Confinement plus myosin-II suppression maximizes heritable loss of chromosomes, as revealed by live-cell ChReporters. J Cell Sci.
Applications
FC, FACS, FLOW PubMed
Puri P et al. (2022). Elevated Protein Kinase A Activity in Stomach Mesenchyme Disrupts Mesenchymal-epithelial Crosstalk and Induces Preneoplasia. Cell Mol Gastroenterol Hepatol.
Applications
IF, Confocal Microscopy PubMed
Le N et al. (2022). Ectopic insert-dependent neuronal expression of GFAP promoter-driven AAV constructs in adult mouse retina. Front Cell Dev Biol.
Applications
IHC, ICC, Histology PubMed
Yao L et al. (2022). Temporal control of PDGFRα regulates the fibroblast-to-myofibroblast transition in wound healing. Cell Rep.
Applications
IF, Confocal Microscopy PubMed
Szabo GG et al. (2022). Ripple-selective GABAergic projection cells in the hippocampus. Neuron.
Applications
IHC, ICC, Histology PubMed
Ademi H et al. (2022). Deciphering the origins and fates of steroidogenic lineages in the mouse testis. Cell Rep.
Applications
IF, Confocal Microscopy PubMed
Weera, MM et al. (2022). Generation of a CRF1-Cre transgenic rat and the role of central amygdala CRF1 cells in nociception and anxiety-like behavior. ELife
Applications
IF, Confocal Microscopy PubMed
Topilko, T et al. (2022). Edinger-Westphal peptidergic neurons enable maternal preparatory nesting. Neuron
Applications
IHC, ICC, Histology PubMed
Serrano, J et al. (2022). Saccharin Stimulates Insulin Secretion Dependent on Sweet Taste Receptor-Induced Activation of PLC Signaling Axis. Biomedicines
Applications
IF, Confocal Microscopy PubMed
Serrano J et al. (2022). Saccharin Stimulates Insulin Secretion Dependent on Sweet Taste Receptor-Induced Activation of PLC Signaling Axis. Biomedicines.
Applications
IF, Confocal Microscopy Source
Hou WH et al. (2022). Inhibitory Fear Memory Engram in the Mouse Central Lateral Amygdala. Cell Press Preprint
Applications
Undefined Source
Hara A et al. (2021). Meflin defines mesenchymal stem cells and/or their early progenitors with multilineage differentiation capacity. Genes Cells.
Applications
IF, Confocal Microscopy PubMed
Okuchi Y et al. (2021). Wnt-modified materials mediate asymmetric stem cell division to direct human osteogenic tissue formation for bone repair. Nat Mater.
Applications
Undefined PubMed
Junyent S et al. (2021). Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells. J Cell Biol.
Applications
IF, Confocal Microscopy PubMed
Zhang W et al. (2021). Targeting KDM4A epigenetically activates tumor-cell-intrinsic immunity by inducing DNA replication stress. Mol Cell.
Applications
Undefined PubMed
Maksymetz J et al. (2021). mGlu1 potentiation enhances prelimbic somatostatin interneuron activity to rescue schizophrenia-like physiological and cognitive deficits. Cell Rep.
Applications
IF, Confocal Microscopy PubMed
Delpech JC et al. (2021). Wolframin-1–expressing neurons in the entorhinal cortex propagate tau to CA1 neurons and impair hippocampal memory in mice. Sci Transl Med.
Applications
IHC, ICC, Histology; Immuno-EM PubMed
Weera MM et al. (2021). Generation and validation of a CRF1:Cre transgenic rat: The role of central amygdala CRF1 in nociception and anxiety-like behavior. bioRxiv Preprint
Applications
IF, Confocal Microscopy Source
Treffy RW et al. (2021). Neuroblastoma differentiation in vivo excludes cranial tumors. Dev Cell.
Applications
Undefined PubMed
Yao L et al. (2021). Fibrosis Without Myofibroblasts Revealed by Genetic Analysis of PDGFRα. Cell Press Preprint
Applications
Undefined Source
Haidey JN et al. (2021). Astrocytes regulate ultra-slow arteriole oscillations via stretch-mediated TRPV4-COX-1 feedback. Cell Rep.
Applications
IHC, ICC, Histology PubMed
Mayere C et al. (2021). Origin, specification and differentiation of a rare supporting-like lineage in the developing mouse gonad. HAL Preprint.
Applications
IHC, ICC, Histology Source
Holt JR et al. (2021). Spatiotemporal dynamics of PIEZO1 localization controls keratinocyte migration during wound healing. Elife.
Applications
IF, Confocal Microscopy PubMed
Ishikura Y et al. (2021). In vitro reconstitution of the whole male germ-cell development from mouse pluripotent stem cells. Cell Stem Cell.
Applications
Undefined PubMed
Uchigashima M. et al. (2020). A Specific Neuroligin3-αNeurexin1 Code Regulates GABAergic Synaptic Function in Mouse Hippocampus. ELife
Applications
IHC, ICC, Histology PubMed
Uchigashima M. et al. (2020). A Specific Neuroligin3-αNeurexin1 Code Regulates GABAergic Synaptic Function in Mouse Hippocampus. ELife
Applications
IF, Confocal Microscopy PubMed
Kim CK, Saxena M, Maharjan K, et al. (2020). Krüppel-like Factor 5 Regulates Stemness, Lineage Specification, and Regeneration of Intestinal Epithelial Stem Cells. Cell Mol Gastroenterol Hepatol.
Applications
IF, Confocal Microscopy; FC, FACS, FLOW PubMed
Kuziel G, Thompson V, D'Amato JV, Arendt LM. (2020). Stromal CCL2 Signaling Promotes Mammary Tumor Fibrosis through Recruitment of Myeloid-Lineage Cells. Cancers (Basel).
Applications
IF, Confocal Microscopy PubMed
Turi GF, Li WK, Chavlis S, et al. (2019). Vasoactive Intestinal Polypeptide-Expressing Interneurons in the Hippocampus Support Goal-Oriented Spatial Learning. Neuron.
Applications
IHC, ICC, Histology PubMed
Lihong Zhan et al. (2019). A Mac2-positive progenitor-like microglial population survives independent of CSF1R signaling in adult mouse brain. Elife.
Applications
IHC, ICC, Histology PubMed
Chen M, Reed RR, Lane AP. (2017). Acute inflammation regulates neuroregeneration through the NF-κB pathway in olfactory epithelium. Proc Natl Acad Sci U S A.
Applications
IHC, ICC, Histology PubMed
Lebrigand, K et al. (2016). Comparative Genomic Analysis of Drechmeria coniospora Reveals Core and Specific Genetic Requirements for Fungal Endoparasitism of Nematodes. PloS Genetics
Applications
WB, IB, PCA PubMed
Clancy, JW et al. (2015). Regulated delivery of molecular cargo to invasive tumour-derived microvesicles. Nature Communications
Applications
WB, IB, PCA PubMed
Related Protocols
Adherent Cell Lysis Protocol
Flow Cytometry (FC) Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol
Certificate of Analysis Lookup
Disclaimer
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
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