Anti-RFP (CHICKEN) Antibody

Cat# 600-901-379

Size : 100ug

Brand : Rockland Immunochemicals

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Specifications for RFP Antibody

Product Details

Anti-RFP (CHICKEN) Antibody - 600-901-379
chicken anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
Chicken
Polyclonal
IgY

Target Details

DsRed  - View All DsRed Products
RFP, rRFP, tdTomato
Recombinant Protein
The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by Western blot.
Q9U6Y8 - UniProtKB

Application Details

ELISA, WB
FC, IF, IHC, Multiplex  - View References
Anti-RFP is designed to detect recombinant RFP. Anti-RFP antibody has been tested by ELISA and Western blot to detect RFP. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by Western blot.

Formulation

1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

Background

Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays such as Western blotting.

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IHC, ICC, Histology
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IHC, ICC, Histology
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IHC, ICC, Histology
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IF, Confocal Microscopy
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FC, FACS, FLOW; IF, Confocal Microscopy
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IHC, ICC, Histology
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IHC, ICC, Histology
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IF, Confocal Microscopy
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IHC, ICC, Histology
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IHC, ICC, Histology
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IHC, ICC, Histology
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IF, Confocal Microscopy
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IF, Confocal Microscopy
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IF, Confocal Microscopy
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IHC, ICC, Histology
PubMed
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IHC, ICC, Histology
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IF, Confocal Microscopy
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IHC, ICC, Histology
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IHC, ICC, Histology
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IF, Confocal Microscopy
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IHC, ICC, Histology
PubMed
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IHC, ICC, Histology
PubMed
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Applications
IHC, ICC, Histology; Multiplex Assay
PubMed
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Applications
IF, Confocal Microscopy
PubMed
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IHC, ICC, Histology
PubMed
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Applications
IF, Confocal Microscopy
PubMed
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Applications
WB, IB, PCA
PubMed
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IHC, ICC, Histology
PubMed
(). Layer I Interneurons Sharpen Sensory Maps during Neonatal Development. Neuron.
Applications
IF, Confocal Microscopy
PubMed
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IHC, ICC, Histology
PubMed
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IF, Confocal Microscopy
PubMed
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IF, Confocal Microscopy
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IF, Confocal Microscopy
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Applications
IF, Confocal Microscopy
PubMed
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Applications
WB, IB, PCA
PubMed
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Applications
IHC, ICC, Histology
PubMed
(). The transcription factor Prox1 is essential for satellite cell differentiation and muscle fibre-type regulation. Nature Communications
Applications
IHC, ICC, Histology
PubMed
(). Comparative Genomic Analysis of Drechmeria coniospora Reveals Core and Specific Genetic Requirements for Fungal Endoparasitism of Nematodes. PloS Genetics
Applications
WB, IB, PCA
PubMed
(). Ubiquitination and proteasomal degradation of ATG12 regulates its proapoptotic activity. Autophagy
Applications
WB, IB, PCA
PubMed
(). Regulated delivery of molecular cargo to invasive tumour-derived microvesicles. Nature Communications
Applications
WB, IB, PCA
PubMed

Related Protocols

Adherent Cell Lysis Protocol
ELISA Protocol
Flow Cytometry (FC) Protocol
Fluorescent Western Blotting Protocol
Heat-induced Antigen Retrieval Protocol
Histone Immunoblotting Protocol
Immunocytochemistry (ICC) Protocol
Immunofluorescence (IF) Protocol
Immunohistochemistry (IHC) Protocol
In-Cell Western (ICW) Protocol
IP-WB with TrueBlot® Protocol
Multi-Lysate Western Blotting Protocol
Nuclear & Cytoplasmic Extract Protocol
Protease-induced Antigen Retrieval Protocol
Sandwich ELISA Protocol for Collagen
Staining Paraffin Sections by PAP Procedure
Suspension Cultured Cell Lysis Protocol
Western Blotting (WB) Protocol

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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