Anti-Human HLA-A, B, C (MHC Class I) - Purified in vivo GOLD™ Functional Grade
Cat# H263-100
Size : 100mg
Brand : Leinco Technologies
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AntiHuman HLAA, B, C (MHC Class I) – Purified in vivo GOLD™ Functional Grade
AntiHuman HLAA, B, C (MHC Class I) – Purified in vivo GOLD™ Functional Grade
Product No.: H263
Clone W6/32 Target HLAA,B,C Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Major Histocompatibility Class I, MHC class I, human leukocyte antigen (HLA) Isotype Mouse IgG2a k Applications B , FC , IHC FF , in vivo , IP , PhenoCycler® , WB |
Antibody DetailsProduct DetailsReactive Species Baboon ⋅ Chimpanzee ⋅ Cynomolgus Monkey ⋅ Feline ⋅ Bovine ⋅ Human Host Species Mouse Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Human tonsil cell membrane Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multistep process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 28°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ 70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 28°C RRIDAB_2737521 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this HLAA,B,C Clone W6/32 antibody for staining cells in flow cytometry is ≤ 2.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for for use in western blotting is 110 μg/ml. Additional Applications Reported In Literature ? B CODEX® IHC FF IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone W6/32 recognizes the human MHC class I molecules HLAA, B, and C. Background HLA antibody, clone W6/32, recognizes the major histocompatibility complex (MHC) class I molecules human leukocyte antigen (HLA)A, HLAB, and HLAC. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45kDa type I transmembrane glycoprotein (αchain or heavy chain) and a 12kDa soluble protein (β2microglobulin, β2M)1,2. The αchain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigenbinding groove and bind to 810 aa peptides derived from cytosolic antigens46. β2M noncovalently associates with α3, which is essential for MHC stability. MHC class I plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as crosspresentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptideMHC class I, which induces CD8 T cell activation and subsequent cellkilling. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11. Antigen Distribution HLAA, B, and C are ubiquitously expressed on nucleated cells. Ligand/Receptor CD3/TCR, CD8 Function Antigen presentation PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity References & Citations1. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):1061825 2. Wieczorek M, et al. (2017) Front Immunol. 8:292 3. Jones EY. (1997) Curr Opin Immunol. 9(1):759 4. Matsumura M, et al. (1992) Science. 257:927–34.10.1126/science.1323878 5. Bouvier M & Wiley DC. (1994) Science. 265:398–402.10.1126/science.8023162 6. Zacharias M & Springer S. (2004) Biophys J. 87:2203–14.10.1529/biophysj.104.044743 7. Cruz FM, et al (2017) Annu Rev Immunol. 35:149176 8. Artyomov MN, et al (2010) Proc Natl Acad Sci USA. 107(39):1691616921 9. Orr MT & Lanier LL. (2010) Cell. 142(6):847856 10. Raulet DH. (1994) Adv Immunol. 55:381421 11. Salcedo M & Ljunggren HG. (1996) Chem Immunol. 64:4458 You might also be interested by the following products:
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